皮肤收缩现象是由于手术CO2激光发射的能量影响组织中的胶原结构和细胞内含水量而发生的。这项研究旨在评估性别,年龄,品种,体重,CO2激光排放模式,和效力影响切口后的皮肤收缩。该研究涉及80只男女狗(N=80),多个品种,用CO2激光进行大手术。基于激光效能(12或15瓦)和发射模式(Superpulse-SP或连续-CT)对受试者进行分组:GSP12、GSP15、GCT12和GCT15。使用手术CO2激光束进行10mm切口,始终采用0.4毫米的焦点,位于距离皮肤表面1毫米处,并始终保持垂直于它,和产生的长度用数字卡尺测量。对于p值<0.05,结果被认为是显著的。GSP12显示最小收缩,而GCT15表现最显著。GCT12,GCT15和GSP12中的男性受试者的收缩少于女性。纯种狗比混合品种的收缩更大。GSP12个体显示与年龄相关的收缩减少(p<0.01),皮肤每年收缩0.09毫米。体重和皮肤收缩趋势显著(p=0.06),每单位重量增加0.02毫米。对于12W的恒定功率,分析了激光光束的发射模式与最终皮肤收缩之间的关系(GSP12vs.GCT12)显示出统计学上的显着差异(p<0.01)。这项研究表明,使用连续模式的激光发射,不管使用的功率,与更高水平的最终皮肤收缩有关。前瞻性注册试验的试验注册编号和注册日期:研究和教学道德委员会(CEIE)的项目批准注册编号,里斯本大学兽医学院(FMV_ULisboa),Lisboa-葡萄牙,N/Refä015/2022。
The skin contraction phenomenon occurs due to the energy emitted by the surgical CO2 LASER affecting the collagen architecture and intracellular water content in tissues. The
study aimed to assess how gender, age, breed, body-weight, CO2 LASER emission mode, and potency influence skin contraction following the incision. The
study involved 80 dogs (N = 80) of both genders, multiple breeds, undergoing major surgery with CO2 LASER. Subjects were grouped based on LASER potency (12 or 15 Watts) and emission mode (Superpulse-SP or Continuous-CT): GSP12, GSP15, GCT12, and GCT15. A 10 mm incision was performed using the surgical CO2 LASER beam, consistently employing a focal point of 0.4 mm, positioned at a distance of 1 mm from the skin surface, and always maintained perpendicular to it, and resulting lengths measured with a digital caliper. Results were considered significant for p-value < 0.05. GSP12 showed minimal contraction, while GCT15 exhibited the most significant. Male subjects in GCT12, GCT15, and GSP12 experienced less contraction than females. Purebred dogs had greater contraction than mixed breeds. GSP12 individuals showed age-related contraction decrease (p < 0.01), with skin contracting by 0.09 mm per year. Weight and skin contraction trended towards significance (p = 0.06), with a 0.02 mm increase per unit weight. For a constant power of 12 W, the analysis of the relationship between the emission mode of the LASER beam and the final skin contraction (GSP12 vs. GCT12) revealed statistically significant differences (p < 0.01). This
study suggests that the use of the Continuous mode of LASER emission, regardless of the power used, is associated with a higher level of final skin contraction.
TRIAL REGISTRATION NUMBER AND DATE OF REGISTRATION FOR PROSPECTIVELY REGISTERED TRIALS: Project approval registration number by the Research and Teaching Ethics Committee (CEIE),Faculty of Veterinary Medicine-University of Lisbon (FMV_ULisboa), Lisboa-Portugal, N/Refª 015/2022.