Lameness, Animal

跛行 ,动物
  • 文章类型: Journal Article
    骶髂关节功能障碍(SID)是在与影响后肢步态和冲动的不良表现相关的马中看到的病症。病情包括疼痛和功能障碍,但缺乏明确的病因和SID是否包括异常关节病理,关节运动异常,区域生物力学功能异常,关节松弛和疼痛,或可能随时间变化的各种组合。由于骶髂关节(SIJ)和周围结构的较深位置限制了触诊的进入,临床评估对马临床医生来说仍然具有挑战性。诊断成像和关节特异性注射。SID没有公认的单一参考标准诊断测试。临床诊断是基于排除后肢跛行的其他原因,连同超声检查的组合,SIJ的闪烁显像和关节周围麻醉。最近的研究强调了针对SIJ的注射缺乏特异性,注射物明显分散到周围结构,包括腰骶关节(LSJ)周围。先进的成像方式,如计算机断层扫描为评估SIJ和周围骨结构的结构和病理提供了希望。然而,有必要提高对骶髂区结构解剖变异的重要性的理解,最近的研究报告了有和没有SID的马群的详细解剖变异。关节的功能评估也存在局限性,这在很大程度上仍然依赖于彻底的临床检查。这篇综述旨在提供SID马诊断的临床方法的最新信息。并考虑挑战和限制。
    Sacroiliac dysfunction (SID) is a condition seen in horses associated with poor performance that affects hind limb gait and impulsion. The condition comprises pain and dysfunction but there lacks clarity around the aetiopathogenesis and whether SID encompasses abnormal joint pathology, abnormal joint movement, abnormal regional biomechanical function, joint laxity and pain, or various combinations of these that may vary over time. Clinical assessment remains challenging for equine clinicians due to the deep location of the sacroiliac joint (SIJ) and surrounding structures which limits access for palpation, diagnostic imaging and joint-specific injection. There is no recognised single reference standard diagnostic test for SID. Clinical diagnosis has been based on ruling out other causes of hind limb lameness, along with combinations of ultrasonography, scintigraphy and periarticular anaesthesia of the SIJ. Recent studies have highlighted the lack of specificity of injections targeting the SIJ, with significant dispersal of injectate into surrounding structures including around the lumbosacral joint (LSJ). Advanced imaging modalities such as computed tomography offers promise for assessment of the structure and pathology of the SIJ and surrounding bony structures. However, there is a need to improve the understanding of the significance of anatomic variation of the sacroiliac region structures, with recent studies reporting detailed anatomic variation in groups of horses with and without SID. There are also limitations around functional assessment of the joint which is still largely reliant on a thorough clinical examination. This review aims to present an update on clinical approaches to the diagnosis of horses with SID, and to consider the challenges and limitations.
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  • 文章类型: Journal Article
    跛行和腿部受伤在整个乳制品行业都是痛苦和普遍的,是一个主要的福利问题。已经有大量的研究集中在调查与跛行和伤害相关的风险因素以及如何预防和治疗它们。这篇叙述性综述的目的是总结牧群水平的患病率估计,危险因素,预防战略,control,以及这些疾病的治疗,以及奶牛场采用跛行和受伤的最佳做法的障碍。在全球奶牛场中,羊群内跛行的患病率相对较高,最近的系统评价估计平均患病率为22.8%。同样,有一个相对较高的流行率,牛群内估计有12%到81%的奶牛受到影响。据报道,膝盖和颈部受伤较不常见;6-43%和1-33%,分别。许多危险因素与跛行的发生率有关,特别是住房(例如,进入牧场,垫层深度,床上用品类型,地板类型,失速设计),管理(例如,隔间清洁度,微调频率,holdingtimes,放养密度),和牛级(例如,身体状况,奇偶校验,受伤的飞节)因素。与hock伤相关的风险因素可以类似地分类为住房(例如,垫层类型和深度,户外通道,客厅类型,失速设计),管理(例如,垫层深度,清洁度),和母牛(例如,奇偶校验,牛奶中的日子,跛行)因素。预防跛行的主要方法包括常规预防和矫正蹄修剪,通过进入牧场或添加橡胶地板来改善蹄子缓冲和牵引力,深层摊位,砂垫层,确保适当的放养密度,减少保持时间,以及经常使用常规的足浴。关于飞节的研究很少,膝盖,和颈部损伤的预防和恢复。许多研究人员得出结论,两者都是外在的(例如,时间,钱,空间)和内在(例如,农民的态度,感知,优先事项,和心态)在解决奶牛场的跛行和伤害方面存在障碍。在跛行和伤害管理方面有许多不同的利益相关者,包括农民,农场员工,兽医,蹄修剪器,营养学家,和其他顾问。解决奶牛跛行和受伤问题必须,因此,考虑到参与的人,因为正是这些人在影响和实施与预防跛行有关的农场决策,治疗,和控制。
    Lameness and leg injuries are both painful and prevalent across the dairy industry, and are a major welfare concern. There has been a considerable amount of research focused on investigating the risk factors associated with lameness and injuries and how they might be prevented and treated. The objectives of this narrative review were to summarize herd-level prevalence estimates, risk factors, strategies for prevention, control, and treatment of these conditions, and the barriers to best practice adoption for lameness and injuries on dairy farms. There is a relatively high within-herd prevalence of lameness on dairy farms globally, with a recent systematic review estimating the mean prevalence at 22.8%. Similarly, there is a relatively high prevalence of hock injuries, with within-herd estimates ranging from 12% to 81% of cows affected. Knee and neck injuries have been reported to be less common, with 6% to 43% and 1% to 33%, respectively. Numerous risk factors have been associated with the incidence of lameness, notably housing (e.g., access to pasture, bedding depth, bedding type, flooring type, stall design), management (e.g., stall cleanliness, frequency of trimming, holding times, stocking density), and cow-level (e.g., body condition, parity, injured hocks) factors. Risk factors associated with hock injuries can be similarly classified into housing (e.g., bedding type and depth, outdoor access, parlor type, stall design), management (e.g., bedding depth, cleanliness), and cow (e.g., parity, days in milk, lameness) factors. Key preventative approaches for lameness include routine preventative and corrective hoof trimming, improving hoof cushioning and traction through access to pasture or adding rubber flooring, deep-bedded stalls, sand bedding, ensuring appropriate stocking densities, reduced holding times, and the frequent use of routine footbaths. Very little research has been conducted on hock, knee, and neck injury prevention and recovery. Numerous researchers have concluded that both extrinsic (e.g., time, money, space) and intrinsic (e.g., farmer attitude, perception, priorities, and mindset) barriers exist to addressing lameness and injuries on dairy farms. There are many diverse stakeholders in lameness and injury management including the farmer, farm staff, veterinarian, hoof trimmer, nutritionist, and other advisors. Addressing dairy cattle lameness and injuries must, therefore, consider the people involved, as it is these people who are influencing and implementing on-farm decisions related to lameness prevention, treatment, and control.
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  • 文章类型: Journal Article
    目的:这项回顾性研究旨在表征和评估应急方法,诊断,管理,治疗,和非特异性犬跛行病例的随访。
    方法:回顾性病例系列,2013年9月25日至2014年9月25日。
    方法:本研究在城市大学教学医院进行。
    方法:在指定的时间范围内非特异性跛行出现在紧急服务机构的狗的连续样本用于识别134例病例。与主人联系以参与后续调查;在数据收集之前死亡的狗被排除在调查之外。对跛行定位的医疗记录进行了审查,跛行的病因,获得的诊断,开处方的药物,和业主建议。
    方法:根据对病历的审查,跛行本地化,跛行的推定来源(联合,软组织,神经学,或骨头),获得的诊断,开处方的药物,并记录了业主的建议。调查数据包括跛行的持续时间,感知对治疗的反应,和活动水平。
    结果:88.8%的跛行病例没有明确诊断。在45.3%的病例中,软组织损伤的推定诊断被分配。单肢跛行比多肢跛行更为普遍。狗接受药物治疗的主人更有可能报告跛行消退(P=0.049)。局部损伤≥1个关节的狗,跛行消退的可能性明显较小(P=0.037)。治疗建议主要是疼痛控制和活动限制。
    结论:非特异性跛行约占城市急症病例的4%。在了解狗跛行的病因时突出临床护理注意事项,代表了在急诊转诊和随访中改善患者护理和成长的机会。
    OBJECTIVE: This retrospective study aimed to characterize and evaluate the emergency approach, diagnosis, management, treatment, and follow-up of nonspecific canine lameness cases.
    METHODS: Retrospective case series from September 25, 2013 to September 25, 2014.
    METHODS: The study was conducted at an urban university teaching hospital.
    METHODS: A consecutive sample of dogs presenting to the emergency service with nonspecific lameness in the designated timeframe was used to identify 134 cases. Owners were contacted to participate in the follow-up survey; dogs that died prior to data collection were excluded from the survey. Medical records were reviewed for lameness localization, etiology of lameness, diagnostics obtained, medications prescribed, and owner recommendations.
    METHODS: Based on review of the medical records, the lameness localization, presumptive source of lameness (joint, soft tissue, neurological, or bone), diagnostics obtained, medications prescribed, and owner recommendations were recorded. Survey data included duration of lameness, perceived response to treatment, and activity level.
    RESULTS: Definitive diagnoses were not assigned in 88.8% of lameness cases. A presumptive diagnosis of soft tissue injury was assigned in 45.3% of cases. Single limb lameness was more prevalent than multiple limb lameness. Owners whose dogs were treated with medication were significantly more likely to report that the lameness resolved (P = 0.049). Dogs with injury localized to ≥1 of the joints were significantly less likely to have resolution of lameness (P = 0.037). Treatment recommendations were predominantly pain control and activity restriction.
    CONCLUSIONS: Nonspecific lameness represents approximately 4% of canine urban emergency cases. Highlighting the points of clinical care considerations in understanding the etiology of lameness in dogs represents an opportunity for improved patient care and growth in emergency referral and follow-up.
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  • 文章类型: Journal Article
    由于非洲大陆不同地区的有利气候条件,蜱是影响小反刍动物的重要经济病原体的主要载体。他们对畜牧业的直接和间接经济损失负责。这篇综述的重点是硬蜱的物种多样性,他们的生物学,绵羊和山羊的蜱传疾病,包括非传染性疾病,以及非洲蜱虫感染的危险因素。此外,我们的综述提供了非洲小反刍动物蜱和蜱传病原体分布的最新情况.观察到属于Hyalomma(Hy)属的硬蜱的几种和亚种,Rhipicephalus(Rh),在该大陆的不同地区发现了Ixodes(I)和Amblyomma(Am)感染小反刍动物。在这些属中,触毛蜱占登记物种的大部分,恰好有27种不同的物种侵染了小反刍动物种群,这些种群由tick的不同发育幼虫和成虫组成。去象形虫,Rh.e.evertsi和Rh。阑尾是所报道的三种最常见的动物。原生动物(Babesia和Theileria)和细菌(Anaplasma,立克次体,埃里希亚,据报道,柯西氏菌和支原体)病原体在几种硬蜱物种和/或小反刍动物宿主中被扩增。此外,蜱瘫痪和跛行是归因于蜱感染的非感染性疾病。Amblyommahebraeum和Rh。glabroscutatum可能导致山羊跛行,而Hy.rufipes是造成美利奴羊相同状况的原因。由于女性Rh释放的神经毒素引起的宿主瘫痪。e.evertsi和I.rubicundus在该大陆有记载。因此,我们主张需要对蜱传病原体(TBP),包括其节肢动物媒介采取综合控制措施,同时进行,以减轻小型反刍动物生产中媒介传播疾病的负担。
    Ticks are leading vectors of economically important pathogens that affect small ruminants due to favourable climatic conditions across different regions of the African continent. They are responsible for both direct and indirect economic losses in the livestock industry. This review focuses on the species diversity of hard ticks, their biology, tick-borne diseases of sheep and goats including non-infectious disease, and risk factors to tick infestation in Africa. Furthermore, our review provides recent updates on distribution of ticks and tick-borne pathogens of small ruminants in Africa. It was observed that several species and subspecies of hard ticks belonging to the genera Hyalomma (Hy), Rhipicephalus (Rh), Ixodes (I) and Amblyomma (Am) were found infesting small ruminants across the different regions of the continent. Of these genera, Rhipicephalus ticks accounts for the majority of the registered species, with exactly 27 different species infesting small ruminant stocks comprising of different developmental instars and adults of the tick. Rhipicephalus decolaratus, Rh. e. evertsi and Rh. appendiculatus were the three most common Rhipicephalus species reported. Both protozoal (Babesia and Theileria) and bacterial (Anaplasma, Rickettsia, Ehrlichia, Coxiella and Mycoplasma) pathogens have being reported to be amplified in several hard tick species and/or small ruminant hosts. Furthermore, tick paralysis and lameness were non-infectious conditions attributed to tick infestations. Amblyomma hebraeum and Rh. glabroscutatum may cause lameness in goats, while Hy. rufipes is responsible for the same condition in Merino sheep. Host paralysis due to a neurotoxin released by female Rh. e. evertsi and I. rubicundus has been documented within the continent. We therefore advocate for the need of integrated control measures against tick-borne pathogens (TBPs) including their arthropod vectors, to be performed simultaneously to ease the burden of vector-borne diseases in small ruminant production.
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  • 文章类型: Journal Article
    客观的步态分析提供了有关健全和跛脚马的运动特征的有价值的信息。由于其高精度和灵敏度,惯性测量单元(IMU)已经在诸如测力板和光学运动捕获(OMC)系统的客观测量技术上获得了普及。IMU是可穿戴传感器,可测量加速度力和角速度,提供了在行走过程中对马步态进行非侵入性和连续监测的可能性,小跑,或在现场条件下慢跑。本叙述综述旨在描述惯性传感器技术并总结其在马步态分析中的作用。使用与惯性传感器及其适用性相关的一般术语搜索了文献,步态分析方法,和跛行评价。基于IMU的方法评估正常步态的功效和性能,检测跛行,骑马者互动分析,以及镇静药物的影响,进行了讨论,并与力板和OMC技术进行了比较。收集的证据表明,基于IMU的传感器系统可以高精度和高精度地监测和量化马的运动,具有与客观测量技术相当或优越的性能。IMU是评估骑马者互动的可靠工具。在马步态分析中观察到的IMU系统的功效和性能值得在该人群中进行进一步研究。特别关注在人类中描述和验证的新技术的潜在实现。
    Objective gait analysis provides valuable information about the locomotion characteristics of sound and lame horses. Due to their high accuracy and sensitivity, inertial measurement units (IMUs) have gained popularity over objective measurement techniques such as force plates and optical motion capture (OMC) systems. IMUs are wearable sensors that measure acceleration forces and angular velocities, providing the possibility of a non-invasive and continuous monitoring of horse gait during walk, trot, or canter during field conditions. The present narrative review aimed to describe the inertial sensor technologies and summarize their role in equine gait analysis. The literature was searched using general terms related to inertial sensors and their applicability, gait analysis methods, and lameness evaluation. The efficacy and performance of IMU-based methods for the assessment of normal gait, detection of lameness, analysis of horse-rider interaction, as well as the influence of sedative drugs, are discussed and compared with force plate and OMC techniques. The collected evidence indicated that IMU-based sensor systems can monitor and quantify horse locomotion with high accuracy and precision, having comparable or superior performance to objective measurement techniques. IMUs are reliable tools for the evaluation of horse-rider interactions. The observed efficacy and performance of IMU systems in equine gait analysis warrant further research in this population, with special focus on the potential implementation of novel techniques described and validated in humans.
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  • 文章类型: Journal Article
    现代肉鸡具有巨大的遗传潜力,可以在完全成熟的年龄之前增加体重,并具有巨大的代谢需求。此外,这使得肉鸡容易感染机会性病原体,这些病原体可能在应激下进入运动器官,引起细菌性软骨坏死和骨髓炎(BCO)。由于肉鸡的快速生长速率以及血管缺血,在骨骼中形成的微骨折和裂隙进一步加速了这种致病性定植。此外,有几种途径可以改变骨骼稳态,如急性期反应,以及内在和外在的细胞死亡途径。相比之下,即使存在引起感染的跛行相关因素,所有受影响的鸟类也可能不会表现出临床跛行。虽然葡萄球菌,大肠杆菌,肠球菌被认为是参与BCO的常见细菌病原体,但也存在其他几种不可培养的细菌。在肠道中维持稳态环境的任何偏差都可能导致细菌通过血液移位,随后病原菌在包括骨骼在内的各个器官中增殖。重要的是减轻血液的生态失调,其类似于肠道中的生态失调。这可以通过补充pro来实现,pre,和合生元,有助于提供一个优生的环境,减少细菌易位,研究BCO的发病率。这篇综述集中在潜在的和新的生物标志物,病理生理机制,BCO的经济意义,免疫机制,以及导致BCO的各种因素。此外,探索了肠道微生物组的作用以及它们的多样性和来自鸡的致密骨头的细胞培养模型在更好地理解BCO中的作用。
    Modern day broilers have a great genetic potential to gain heavy bodyweights with a huge metabolic demand prior to their fully mature ages. Moreover, this made the broilers prone to opportunistic pathogens which may enter the locomotory organs under stress causing bacterial chondronecrosis and osteomyelitis (BCO). Such pathogenic colonization is further accelerated by microfractures and clefts that are formed in the bones due to rapid growth rate of the broilers along with ischemia of blood vessels. Furthermore, there are several pathways which alter bone homeostasis like acute phase response, and intrinsic and extrinsic cell death pathways. In contrast, all the affected birds may not exhibit clinical lameness even with the presence of lameness associated factors causing infection. Although Staphylococcus, E. coli, and Enterococcus are considered as common bacterial pathogens involved in BCO, but there exist several other non-culturable bacteria. Any deviation from maintaining a homeostatic environment in the gut might lead to bacterial translocation through blood followed by proliferation of pathogenic bacteria in respective organs including bones. It is important to alleviate dysbiosis of the blood which is analogous to dysbiosis in the gut. This can be achieved by supplementing pro, pre, and synbiotics which helps in providing a eubiotic environment abating the bacterial translocation that was studied to the incidence of BCO. This review focused on potential and novel biomarkers, pathophysiological mechanism, the economic significance of BCO, immune mechanisms, and miscellaneous factors causing BCO. In addition, the role of gut microbiomes along with their diversity and cell culture models from compact bones of chicken in better understanding of BCO were explored.
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  • 文章类型: Review
    The purpose of this report is to demonstrate that radiographic evaluation of the canine shoulder joint alone is not sensitive enough to detect migrated osteochondral fragments within the biceps tendon sheath, as a sequela to osteochondrosis dissecans of the caudal humeral head. A 6-months-old, male, 35 kg Hovawart was referred due to chronic intermittent lameness on the left forelimb. Survey radiographs revealed a semilunar radiolucency surrounded by a moderately sclerotic rim at the caudal aspect of the left humeral head, referred to as osteochondrosis dissecans. However, only computed tomography combined with ultrasonography could clearly confirm a dislodged osteochondral fragment within the left biceps tendon sheath and a consequent tenosynovitis. Arthroscopic treatment on the clinically affected left forelimb followed by an additional approach over the left biceps tendon sheath to remove the migrated fragment resulted in a complete remission of the lameness until the last follow-up one year after surgery. In our opinion, computed tomography should be applied in the medical work up of canine shoulder osteochondrosis (OC) as standard. Combined with ultrasonography, it can further aid in complete evaluation of the shoulder joint and reliable exclusion of displaced osteochondral fragments, which might also be missed during arthroscopy when located too far distally.
    Ziel dieses Fallberichts ist es, zu zeigen, dass die Röntgenuntersuchung des Schultergelenks allein nicht sensitiv genug ist, um dislozierte Fragmente in die Bizepssehnenscheide als Folge einer Osteochondrosis dissecans (OCD) Läsion am kaudalen Humeruskopf bei Hunden zu diagnostizieren. Ein 6 Monate alter, 35 kg schwerer, männlicher Hovawart wurde aufgrund einer chronisch intermittierenden Lahmheit an der linken Vordergliedmaße überwiesen. Auf den Röntgenaufnahmen zeigte sich eine semilunare Radioluzenz mit sklerotischem Randsaum am linken kaudalen Humeruskopf, typisch für eine osteochondrale Läsion. Jedoch konnte nur durch die Kombination von Computertomografie und Sonografie ein disloziertes osteochondrales Fragment in der linken Bizepssehnenscheide und die daraus resultierende Tenosynovitis eindeutig bestätigt werden. Eine chirurgische Versorgung mittels Arthroskopie, gefolgt von einem Zugang über der linken Bizepssehnenscheide zur Entfernung des migrierten Fragments, führte zu einer vollständigen Remission der Lahmheit bis zur letzten Nachuntersuchung ein Jahr postoperativ. Unserer Meinung nach sollte die Computertomografie als Standard in der Abklärung einer OCD des Schultergelenks bei Hunden eingesetzt werden. In Kombination mit einer Sonografie kann sie zur vollständigen Beurteilung des Schultergelenks und zum zuverlässigen Ausschluss dislozierter osteochondraler Fragmente beitragen, welche auch in der Arthroskopie übersehen werden können.
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  • 文章类型: Journal Article
    奶牛跛行对动物福利和生产经济有重大负面影响。虽然以前的研究已经评估了单国家跛行的患病率,本文献综述是对全球奶牛跛行患病率的首次综述。这篇文献综述确定了53项研究,报告了代表性奶牛样本中跛行的患病率,并满足了一些指定的纳入标准(例如,至少有10个牛群和200头牛,并由训练有素的观察员进行运动评分)。共有来自3945个牧群的414,950头牛被纳入这53项研究,跨越30年(1989-2020年),包括来自六大洲的牛群,大部分来自欧洲和北美。在整个研究中,跛行的平均患病率(通常定义为1-5量表上的3-5分)为22.8%,中位数为22.0%,研究之间的范围为5.1%至45%,群体范围从0%到88%。严重跛脚奶牛的平均患病率(通常定义为1-5量表上的4-5分)为7.4%,中位数为6.5%,研究范围为1.8%至21.2%,羊群范围从0%到65%。随着时间的推移,看起来跛行的患病率变化很小。在53项研究中使用了几种不同的运动评分系统和(严重)跛行的定义。这可能影响了报告的跛行患病率。对牛群和奶牛进行采样,纳入标准和代表性也有不同的研究。这篇综述为未来获取奶牛跛行信息提供了建议,并确定了潜在的知识差距。
    Lameness in dairy cows has major negative impacts on animal welfare and production economy. While previous studies have evaluated the prevalence of lameness in single countries, the present literature review is the first overview of the prevalence of lameness in dairy cows globally. This literature review identified 53 studies reporting prevalence of lameness among representative samples of dairy cows and fulfilling a number of specified inclusion criteria (e.g., at least 10 herds and 200 cows, and locomotion scoring by trained observers). A total of 414,950 cows from 3945 herds were included in these 53 studies, which spanned a 30-year period (1989-2020) and included herds from six continents, with the majority from Europe and North America. Across the studies, the mean prevalence of lameness (typically defined as score 3-5 on a 1-5 scale) was 22.8% with a median of 22.0% and a range between studies from 5.1% to 45%, and a within herd range from 0% to 88%. The mean prevalence of severely lame cows (typically defined as score 4-5 on a 1-5 scale) was 7.0% with a median of 6.5% and a range between studies from 1.8% to 21.2%, and a within herd range from 0% to 65%. Over time, it appears that the prevalence of lameness has changed very little. Several different locomotion scoring systems and definitions of (severe) lameness were used across the 53 studies, and this may have affected the reported lameness prevalence. Sampling of herds and cows, inclusion criteria and representativeness also differed between studies. This review offers recommendations for the future capture of information on lameness in dairy cows and identifies potential knowledge gaps.
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  • Reoviral-induced tenosynovitis/viral arthritis is an economically significant disease of poultry. Affected birds present with lameness, unilateral or bilateral swollen hock joints or shanks, and/or reluctance to move. In severe cases, rupture of the gastrocnemius or digital flexor tendons may occur, and significant culling may be necessary. Historically, vaccination with a combination of modified live and inactivated vaccines has successfully controlled disease. Proper vaccination reduced vertical transmission and provided maternal-derived antibodies to progeny to protect against disease, at an age when they were most susceptible. Starting in 2011-2012, an increased incidence of tenosynovitis/viral arthritis was observed in chickens and turkeys. In chickens, progeny from reovirus-vaccinated breeders were affected, suggesting commercial vaccines did not provide adequate protection against disease. In turkeys, clinical disease was primarily in males, although females can also be affected. The most significant signs were observed around 14-16 wks of age and include reluctance to move, lameness, and limping on one or both legs. The incidence of tenosynovitis/viral arthritis presently remains high. Reoviruses isolated from clinical cases are genetically and antigenically characterized as variants, meaning they are different from vaccine strains. Characterization of the field isolates reveals multiple new genotypes and serotypes that are significantly different from commercial vaccines and each other. In 2012, a single prevalent virus was isolated from a majority of the cases submitted to the Poultry Diagnostic and Research Center at the University of Georgia. Genetic characterization of the σC protein revealed the early isolates belonged to genetic cluster (GC) 5. Soon after the initial identification of the GC5 variant reovirus, many broiler companies incorporated these isolates from their farms into their autogenous vaccines and continue to do so today. The incidence of GC5 field isolates has decreased significantly, likely because of the widespread use of the isolates in autogenous vaccines. Unfortunately, variant reoviruses belonging to multiple GCs have emerged, despite inclusion of these isolates in autogenous vaccines. In this review, an overview of nomenclature, sample collection, and diagnostic testing will be covered, and a summary of variant reoviruses isolated from clinical cases of tenosynovitis/viral arthritis over the past 10 yrs will be provided.
    Estudio recapitulativo- Reovirus aviares de casos clínicos de tenosinovitis: una descripción general de los enfoques de diagnóstico y una revisión de 10 años de aislamientos y caracterización genética. La tenosinovitis/artritis viral inducida por reovirus es una enfermedad económicamente significativa de la avicultura. Las aves afectadas presentan cojera, articulaciones de corvejones o patas inflamadas unilateral o bilateralmente y/o renuencia a moverse. En casos severos, puede ocurrir la ruptura de los tendones del gastrocnemio o del flexor digital, y puede ser necesario una eliminación de aves afectadas significativa. Históricamente, la vacunación con una combinación de vacunas vivas modificadas e inactivadas ha controlado con éxito la enfermedad. La vacunación adecuada redujo la transmisión vertical y proporcionó anticuerpos derivados de las reproductoras a la progenie para protegerlos contra la enfermedad, a una edad en la que eran más susceptibles. A partir de los años 2011-2012, se observó una mayor incidencia de tenosinovitis/artritis viral en pollos y pavos. En los pollos, la progenie de reproductores vacunados con reovirus se vio afectada, lo que sugiere que las vacunas comerciales no brindaron una protección adecuada contra la enfermedad. En pavos, la enfermedad clínica fue principalmente en machos, aunque las hembras también pueden verse afectadas. Los signos más significativos se observaron alrededor de las 14 a 16 semanas de edad e incluyen renuencia a moverse y cojera en una o ambas piernas. La incidencia de tenosinovitis/artritis viral actualmente sigue siendo alta. Los reovirus aislados de casos clínicos se caracterizan genética y antigénicamente como variantes, lo que significa que son diferentes de las cepas vacunales. La caracterización de los aislamientos de campo revela múltiples genotipos y serotipos nuevos que son significativamente diferentes de las vacunas comerciales y entre sí. En 2012, se aisló un solo virus prevalente de la mayoría de los casos presentados al Centro de Investigación y Diagnóstico Avícola de la Universidad de Georgia. La caracterización genética de la proteína sigma C reveló que los primeros aislamientos pertenecían al grupo genético 5 (GC5). Poco después de la identificación inicial de la variante GC5 del reovirus, muchas empresas de pollos de engorde incorporaron estos aislamientos de sus granjas en sus vacunas autógenas y continúan haciéndolo en la actualidad. La incidencia de aislamientos de campo de GC5 ha disminuido significativamente, probablemente debido al uso generalizado de los aislamientos en vacunas autógenas. Desafortunadamente, han surgido variantes de reovirus que pertenecen a múltiples grupos genéticos, a pesar de la inclusión de estos aislados en vacunas autógenas. En esta revisión, se cubrirá una descripción general de la nomenclatura, la recolección de muestras y las pruebas de diagnóstico, y se brindará un resumen de las variantes de reovirus aisladas de casos clínicos de tenosinovitis/artritis viral durante los últimos 10 años.
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  • 文章类型: Journal Article
    在过去的二十年中,使用技术的定量牛步态分析取得了显着进展。然而,使用视觉运动评分进行步态评估的主观方法仍然是主要的农场和实验方法。这篇综述的目的是绘制定量牛步态分析的研究趋势,并探索用于测量步态生物力学参数的技术。根据PRISMA指南进行范围界定文献综述。基于PICO框架的搜索算法生成了三个组件-牛,步态,和技术-实现我们的目标。搜索了三个在线数据库,以查找2000年1月至2020年6月发布的原创作品。然后进行两步筛选过程,从基于纳入标准的文章标题和摘要审查开始。剩下的125篇文章随后进行了全文评估,最终产生82篇文章。研究目标的主题分析导致了四个主要的研究主题:步态/爪生物力学,跛行检测,干预/比较,和系统开发。在四个主题中,跛行检测(55%的研究)是技术使用的最常见原因。在文献中确定使用了三种主要技术:力和压力平台(FPP),基于视觉的系统(VB),和加速度计。FPP是第一个也是最受欢迎的评估牛步态的技术,在58.5%的研究中使用。它们包括力量平台,压力测绘系统,和重量分配平台。第二大应用技术是VB(占研究的34.1%),主要由视频分析和图像处理系统组成。加速计,另一种测量步态特征的技术方法,在14.6%的研究中使用。总之,对自动跛行检测的强烈需求影响了定量步态分析技术的发展道路。在新兴技术中,深度学习和可穿戴传感器(例如,加速度计)似乎是最有前途的选择。然而,虽然取得了进展,需要更多的研究来开发更准确的,实用,和用户友好的技术。
    Quantitative bovine gait analysis using technology has evolved significantly over the last two decades. However, subjective methods of gait assessment using visual locomotion scoring remain the primary on-farm and experimental approach. The objective of this review is to map research trends in quantitative bovine gait analysis and to explore the technologies that have been utilized to measure biomechanical parameters of gait. A scoping literature review was conducted according to PRISMA guidelines. A search algorithm based on PICO framework generated three components-bovine, gait, and technology-to address our objectives. Three online databases were searched for original work published from January 2000 to June 2020. A two-step screening process was then conducted, starting with the review of article titles and abstracts based on inclusion criteria. A remaining 125 articles then underwent a full-text assessment, resulting in 82 final articles. Thematic analysis of research aims resulted in four major themes among the studies: gait/claw biomechanics, lameness detection, intervention/comparison, and system development. Of the 4 themes, lameness detection (55% of studies) was the most common reason for technology use. Within the literature identified three main technologies were used: force and pressure platforms (FPP), vision-based systems (VB), and accelerometers. FPP were the first and most popular technologies to evaluate bovine gait and were used in 58.5% of studies. They include force platforms, pressure mapping systems, and weight distribution platforms. The second most applied technology was VB (34.1% of studies), which predominately consists of video analysis and image processing systems. Accelerometers, another technological method to measure gait characteristics, were used in 14.6% of studies. In sum, the strong demand for automatic lameness detection influenced the path of development for quantitative gait analysis technologies. Among emergent technologies, deep learning and wearable sensors (e.g., accelerometers) appear to be the most promising options. However, although progress has been made, more research is needed to develop more accurate, practical, and user-friendly technologies.
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