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OBJECTIVE: To investigate the microbiological status of certain risk areas in the dental technology laboratory, namely pumice slurry, impression agar and curing water baths.
METHODS: Samples were inoculated onto selective and non-selective media. Resultant fungal and bacterial colonies were counted and identified to genus or species level.
RESULTS: Pumice slurry freshly made up using disinfectant was free from contamination, but colony counts approached 10(9) g-1 after 3 days\' use. Impression agar was free from contamination, probably because of its method of preparation and maintenance, which appears to achieve sterility. Staphylococci were most commonly isolated from curing water baths (and from the air). Candida spp. and other yeasts were common in pumice slurry.
CONCLUSIONS: Personnel and managers should be aware of the potential cross-contamination hazards posed by the presence of a range of opportunist pathogens in dental technology laboratories.

Infection of human and rabbit cells with cell-free HTLV-I was studied by PCR analysis. Both human and rabbit PBL were infected similarly by cell-free virus of both human and rabbit cell origin. Cells were infected with the cell-free virus without prior treatment and regardless of the concentration of the culture supernatant containing the virus. Human and rabbit cell lines were also infected similarly by the cell-free virus, the proviral DNA persisting for more than two months. The culture supernatants of HTLV-I-producing cells could thus be a potential cause of laboratory infections.

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Enquiry by questionnaire showed that the attack rate of hepatitis in clinical laboratory staff in Britain had fallen to 35 per 100,000 person-years in 1975--76 from 111 in 1970--72 and 143 in 1973--74. This suggests that increased awareness and application of safety precautions have been effective.

Infectious adenovirus type 5 and coxsackievirus type B5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees C for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin G. Lipid-containing herpes simplex virus type 1 and respiratory syncytial virus were inactivated by acetone, and infectious virus could not be recovered at any stage in the fluorescent antibody test. Fixation in acetone at 56 degrees C destroyed the infectivity of adenovirus 5 and coxsackievirus B5 within 30 min, but no adverse effect on the antigenic determinants of either virus occurred until after 60 min, thus demonstrating that these antigens can be utilized without the hazard of infectious virus.