精神分裂症(SCZ)是一种使人衰弱的疾病,破坏性,和慢性精神障碍,影响大约百分之一的人口。精神病学的诊断是基于患者对他/她的症状的描述,面试官的观察,随着时间的推移,疾病的历史,以及对治疗的反应。所有这些数据测量基于表型的功能。但是,对这种复杂疾病的准确诊断似乎必须基于有效和可靠的因素。在本研究中,基因选择基于γ-氨基丁酸(GABA)在SCZ精神病理学和血液中表达中的可能作用。我们评估了Na+-K+-Cl-协同转运蛋白1(NKCC1)和K+-Cl-协同转运蛋白2(KCC2)基因的关联,SCZ组的NKCC1/KCC2比率以及阳性和阴性综合征量表(PANSS)和简短精神病学评定量表(BPRS)评分。通过使用实时PCR(RT-PCR),本研究是首次尝试探索SCZ患者外周血中NKCC1和KCC2mRNA水平的表达水平及其相对表达。我们的结果表明,NKCC1与KCC2mRNA的比率显着增加(但基于阈值[ΔCt]的δ周期显着降低),而不是对照组(p=0.045),并且在男性样本病例中也更高,而不是男性对照(p=0.016)。在女性样本中,我们发现病例和对照参与者之间有显著效应的趋势(p=0.075).我们还发现NKCC1和KCC2基因的mRNA和NKCC1/KCC2mRNA比率与阳性和阴性综合征量表(PANSS)和简短的精神病学评定量表(BPRS)评分之间存在统计学上的显着关联。
Schizophrenia (SCZ) is a debilitating, destructive, and chronic mental disorder and affects approximately one percent of the human population. Diagnosis in psychiatry is based on the patient\'s descriptions of his/her symptoms, interviewer\'s observations, history of disorder over time, and response to treatment. All of these data measure phenotype-based functions. But it appears that accurate diagnosis of such a complex disorder must be based on valid and reliable factors. In the present study, gene selection was based on the possible role of γ-aminobutyric acid (GABA) in psychopathology of SCZ and expression in blood. We evaluated the association of Na+-K+-Cl- co-transporter 1 (NKCC1) and K+-Cl- co-transporter 2 (
KCC2) genes\' messenger ribonucleic acid (mRNA) levels, and also the NKCC1/KCC2 ratio with positive and negative syndrome scale (PANSS) and brief psychiatric rating scale (BPRS) scores in an SCZ group. By using real-time PCR (RT-PCR), the present study is the first attempt to explore levels of NKCC1 and
KCC2 expression at mRNA level and their relative expression in human peripheral blood of patients with SCZ. Our results showed that the NKCC1 to
KCC2 mRNA ratio is significantly increased (but based on the delta cycle of threshold [∆Ct] is significantly lower) in the total sample of cases rather than controls (p = 0.045) and also higher in male sample cases rather than male controls (p = 0.016). In female samples, we found a trend toward a significant effect between the
case and control participants (p = 0.075). We also found statistically significant association between mRNA of NKCC1 and
KCC2 genes and NKCC1/
KCC2 mRNA ratio with the positive and negative syndrome scale (PANSS) and brief psychiatric rating scale (BPRS) scores.