■缺血性脑卒中是一种常见疾病,对人类健康构成严重威胁。丁酸是肠道微生物的重要代谢产物。最近的研究表明,丁酸盐在脑缺血损伤的动物模型中具有明显的保护作用。
■本研究的目的是通过荟萃分析评估丁酸盐对缺血性脑卒中的保护作用,旨在为丁酸盐在脑缺血患者中的临床应用提供科学依据。
■对2024年1月23日之前发表的所有相关研究进行了系统搜索,WebofScience,科克伦图书馆,和Embase。使用Syrcle的偏倚风险工具进行动物研究评估方法学质量。使用RevMan5.3软件分析数据。
■共纳入9项研究,与对照组相比,丁酸盐显著增加脑内BDNF水平(SMD=2.33,95CI=[1.20,3.47],p<0.005)和P-Akt表达(SMD=3.53,95%CI=[0.97,6.10],p<0.05)。丁酸酯还降低了大脑中的IL-β水平(SMD=-2.02,95%CI=[-3.22,-0.81],p<0.005),TNF-α水平(SMD=-0.86,95%CI=[-1.60,-0.12],p<0.05),和外周血IL-1β水平(SMD=-2.10,95CI=[-3.59,-0.61],p<0.05)。此外,丁酸盐减少脑梗死体积(MD=-11.29,95CI=[-17.03,-5.54],p<0.05),mNSS评分(MD=-2.86,95CI=[-4.12,-1.60],p<0.005),足断层评分(MD=-7.59,95CI=[-9.83,-5,35],p<0.005),和莫里斯水迷宫时间(SMD=-2.49,95CI=[-4.42,-0.55],p<0.05)。
■这项研究的结果表明,丁酸盐在动物模型中对缺血性脑卒中具有保护作用,其机制与减轻炎症反应和抑制细胞凋亡有关。为未来丁酸盐治疗缺血性卒中的临床发展提供了循证依据。
■https://www.crd.约克。AC.英国/PROSPERO/,CRD42023482844。
UNASSIGNED: Cerebral ischaemic stroke is a common disease that poses a serious threat to human health. Butyrate is an important metabolite of intestinal microorganisms. Recent studies have shown that butyrate has a significant protective effect in animal models of cerebral ischaemic injury.
UNASSIGNED: The aim of this study was to evaluate the protective effect of butyrate on cerebral ischaemic stroke by meta-analysis, aiming to provide a scientific basis for the clinical application of butyrate in patients with cerebral ischaemia.
UNASSIGNED: A systematic search was conducted for all relevant studies published before 23 January 2024, in PubMed, Web of Science, Cochrane Library, and Embase. Methodological quality was assessed using Syrcle\'s risk of bias tool for animal studies. Data were analysed using Rev Man 5.3 software.
UNASSIGNED: A total of nine studies were included, and compared with controls, butyrate significantly increased BDNF levels in the brain (SMD = 2.33, 95%CI = [1.20, 3.47], p < 0.005) and P-Akt expression (SMD = 3.53, 95% CI = [0.97, 6.10], p < 0.05). Butyrate also decreased IL-β levels in the brain (SMD = -2.02, 95% CI = [-3.22, -0.81], p < 0.005), TNF-α levels (SMD = -0.86, 95% CI = [-1.60, -0.12], p < 0.05), and peripheral vascular IL-1β levels (SMD = -2.10, 95%CI = [-3.59, -0.61], p < 0.05). In addition, butyrate reduced cerebral infarct volume (MD = -11.29, 95%CI = [-17.03, -5.54], p < 0.05), mNSS score (MD = -2.86, 95%CI = [-4.12, -1.60], p < 0.005), foot fault score (MD = -7.59, 95%CI = [-9.83, -5, 35], p < 0.005), and Morris water maze time (SMD = -2.49, 95%CI = [-4.42, -0.55], p < 0.05).
UNASSIGNED: The results of this study indicate that butyrate has a protective effect on cerebral ischaemic stroke in animal models, and the mechanism is related to reducing inflammation and inhibiting apoptosis. It provides an evidence-based basis for the future clinical development of butyrate in the treatment of ischaemic stroke.
UNASSIGNED: https://www.crd.york.ac.uk/PROSPERO/, CRD42023482844.