静脉注射吸毒者(IDU)中HIV-1传播的特征是遗传多重性高,这表明阻断HIV-1感染面临更大的挑战。我们调查了新疆22例HIV-1早期感染的IDUs通过单基因组测序(SGS)获得的全长gp160基因的749个序列,中国西北,并产生了传播和创始人病毒(T/F病毒)共有序列(IDU。CON).T/F病毒被分类为CRF07_BC亚型并预测为CCR5嗜性病毒。IDU的可变区(V1、V2和V4环)。与异性恋T/F病毒共有序列(HSX。CON)和同性恋T/F病毒共有序列(MSM。CON).在IDU中发现了总共26个N-连接的糖基化位点。CON序列,比MSM少。CON和HSX。CON.来自注射吸毒者的T/F病毒的特征突出了病毒在传播时或在系统传播之前的早期感染中的遗传组成和复杂性,对于开发有效的HIV-1预防方法非常重要。包括疫苗。
HIV-1 transmission in intravenous drug users (IDUs) has been characterized by high genetic multiplicity and suggests a greater challenge for HIV-1 infection blocking. We investigated a total of 749 sequences of full-length gp160 gene obtained by single genome sequencing (SGS) from 22 HIV-1 early infected IDUs in Xinjiang province, northwest China, and generated a transmitted and founder virus (T/F virus)
consensus sequence (IDU.CON). The T/F virus was classified as subtype CRF07_BC and predicted to be CCR5-tropic virus. The variable region (V1, V2, and V4 loop) of IDU.CON showed length variation compared with the heterosexual T/F virus
consensus sequence (HSX.CON) and homosexual T/F virus
consensus sequence (MSM.CON). A total of 26 N-linked glycosylation sites were discovered in the IDU.CON sequence, which is less than that of MSM.CON and HSX.CON. Characterization of T/F virus from IDUs highlights the genetic make-up and complexity of virus near the moment of transmission or in early infection preceding systemic dissemination and is important toward the development of an effective HIV-1 preventive methods, including vaccines.