植物和动物肽的研究已经引起了极大的关注,但是黄粉虫肽的功能特性缺乏研究,特别是关于它们对辐射损伤的潜在缓解作用和潜在机制。本研究旨在探讨黄粉虫肽对辐射损伤的保护作用。小鼠分为五组:正常,辐射模型,和低,medium-,和高剂量黄粉虫肽(TMP)组(0.15g/kgBW,0.30g/kgBW,和0.60g/kgBW)。各种参数,如血细胞计数,骨髓DNA含量,免疫器官指数,血清D-乳酸水平,二胺氧化酶(DAO),内毒素(LPS),在γ射线照射后3天和15天评估炎症因子。此外,通过H&E染色检查肠组织形态,RT-qPCR实验分析肠道炎症因子的表达,免疫组织化学用于评估肠道中紧密连接蛋白ZO-1和Occludin的表达。结果表明,高剂量TMP显着增强了辐射暴露后小鼠的造血系统功能,导致脾指数增加,胸腺指数,血细胞计数,和骨髓DNA产生(p<0.05)。此外,TMP改善了肠屏障完整性并降低了肠通透性。机制见解表明,这些肽可能通过下调炎症因子TNF-α的基因表达来保护肠屏障功能,IL-1β,和IL-6,同时上调紧密连接蛋白ZO-1和Occludin的表达(p<0.05)。总的来说,补充TMP通过增强造血系统和肠道屏障来减轻辐射引起的肠道损伤,为进一步研究这些肽对电离辐射的保护作用的潜在机制提供了有价值的见解。
Research on plant and animal peptides has garnered significant attention, but there is a lack of studies on the functional properties of Tenebrio molitor peptides, particularly in relation to their potential mitigating effect on radiation damage and the underlying mechanisms. This
study aims to explore the protective effects of Tenebrio molitor peptides against radiation-induced damage. Mice were divided into five groups: normal, radiation model, and low-, medium-, and high-dose Tenebrio molitor peptide (TMP) groups (0.15 g per kg BW, 0.30 g per kg BW, and 0.60 g per kg BW). Various parameters such as blood cell counts, bone marrow DNA content, immune organ indices, serum levels of D-lactic acid, diamine oxidase (DAO), endotoxin (LPS), and inflammatory factors were assessed at 3 and 15 days post gamma irradiation. Additionally, the intestinal tissue morphology was examined through H&E staining, RT-qPCR experiments were conducted to analyze the expression of inflammatory factors in the intestine, and immunohistochemistry was utilized to evaluate the expression of tight junction proteins ZO-1 and Occludin in the intestine. The findings revealed that high-dose TMP significantly enhanced the hematopoietic system function in mice post radiation exposure, leading to increased spleen index, thymus index, blood cell counts, and bone marrow DNA production (p < 0.05). Moreover, TMP improved the intestinal barrier integrity and reduced the intestinal permeability. Mechanistic insights suggested that these peptides may safeguard intestinal barrier function by downregulating the gene expression of inflammatory factors TNF-α, IL-1β, and IL-6, while upregulating the expression of tight junction proteins ZO-1 and Occludin (p < 0.05). Overall, supplementation with TMP mitigates radiation-induced intestinal damage by enhancing the hematopoietic system and the intestinal barrier, offering valuable insights for further investigations into the mechanisms underlying the protective effects of these peptides against ionizing radiation.