背景:细胞因子在人体的生理和病理免疫反应中都是至关重要的。这项研究利用流式细胞术测量血浆白细胞介素-2(IL-2)的水平,白细胞介素-4(IL-4),白细胞介素-5(IL-5)和白细胞介素-17A(IL-17A),并建立其参考区间,旨在为临床疾病的诊断和治疗提供数据支持。
方法:根据纳入和排除标准,从2023年1月至2023年6月,本研究共纳入728名参考个体.Kolmogorov-Smirnov试验用于分析血浆IL-2,IL-4,IL-5和IL-17A的分布。根据C28-A3和WS/T402-2012指南,通过单侧百分位法(第95百分位数)建立血浆IL-2,IL-4,IL-5和IL-17A的参考区间。
结果:在这项研究中,血浆IL-2、IL-4、IL-5和IL-17A水平呈非正态分布。健康成人血浆IL-2、IL-4、IL-5和IL-17A浓度在不同性别和年龄之间差异均无统计学意义(均P>0.05)。因此,所有参考个体被合并为一组,并通过流式细胞术建立血浆IL-2,IL-4,IL-5和IL-17的参考间隔(IL-2≤10.25pg/mL,IL-4≤9.87pg/mL,IL-5≤3.36pg/mL,IL-17A≤9.46pg/mL)。
结论:我们首先建立了中国东部江苏地区单中心人群健康成人血浆IL-2、IL-4、IL-5和IL-17A的参考区间。为评估人体免疫状态和临床疾病的诊治提供重要的参考价值。
Cytokines are of utmost importance in both the physiological and pathological immune responses of the human body. This
study utilized flow cytometry to measure the levels of plasma interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5) and interleukin-17A (IL-17A) and established their reference intervals, aiming to provide data support for the diagnosis and treatment of clinical diseases.
According to the inclusion and exclusion criteria, a total of 728 reference individuals were included in this
study from January 2023 to June 2023. The Kolmogorov-Smirnov test was used to analyse the distributions of plasma IL-2, IL-4, IL-5 and IL-17A. The reference intervals of plasma IL-2, IL-4, IL-5 and IL-17A were established by the unilateral percentile method (95th percentile) based on the guidelines of C28-A 3 and WS/T 402-2012.
In this
study, the levels of plasma IL-2, IL-4, IL-5 and IL-17A were nonnormally distributed. The concentrations of plasma IL-2, IL-4, IL-5 and IL-17A in healthy adults were not significantly different by sex or age (all P > 0.05). Therefore, all the reference individuals were combined into one group, and the reference intervals of plasma IL-2, IL-4, IL-5 and IL-17 were established by flow cytometry (IL-2 ≤ 10.25 pg/mL, IL-4 ≤ 9.87 pg/mL, IL-5 ≤ 3.36 pg/mL and IL-17A ≤ 9.46 pg/mL).
We first established the reference intervals of plasma IL-2, IL-4, IL-5 and IL-17A in healthy adults based on a single-center population in the Jiangsu region in eastern China, which will provide an important reference value for evaluating human immune status and the diagnosis and treatment of clinical diseases.