目的:白细胞介素-17A(IL-17A)基因多态性与多种癌症类型相关,包括结直肠癌(CRC)。然而,以前没有在孟加拉国人群中进行过评估这种关联的研究.我们的研究旨在发现两种IL-17A变体(rs10484879C/A和rs3748067G/A)与CRC易感性之间的关联。
方法:这项回顾性病例对照研究包括292例CRC患者,288岁,性别,和BMI匹配健康志愿者。两种变体的基因分型均通过四引物ARMS-PCR方法进行,结果采用SPSS软件包(25.0版)进行分析。
结果:Logistic回归分析表明,在IL-17Ars10484879多态性的情况下,AC和AA基因型携带者显示CRC发展的风险显着增加了2.44和3.27倍(分别为OR=2.44,P=.0008和OR=3.27,P=.0133)。还观察到AC+AA基因型的显著关联(OR=2.58,P=0.0001)。再一次,过度显性和等位基因模型显示与CRC风险有统计学显著关联(OR=2.13,P=.0035和OR=2.22,P=.001).对于rs3748067多态性,AG和AA基因型携带者的CRC风险分别为2.30和2.45倍(OR=2.30,P=0.005和OR=2.45,P=0.031)。AG+AA基因型也观察到统计学上显著的关联(OR=2.35,P=.001),超显性模型(OR=2.05,P=0.014),等位基因模型(OR=2.11,P=.0004)。
结论:本研究强调IL-17Ars1048484879和rs3748067多态性可能与CRC发生有关。然而,更大样本的进一步功能研究可能会显示更多具有统计学意义的结果.
OBJECTIVE: Interleukin-17A (IL-17A) genetic polymorphisms are associated with multiple cancer types, including colorectal cancer (CRC). However, no previous study was performed in the Bangladeshi population to evaluate the association. Our study aimed to find the association between two IL-17A variants (rs10484879 C/A and rs3748067 G/A) and susceptibility of CRC.
METHODS: This retrospective
case-control study comprised 292 CRC patients and 288 age, sex, and BMI matched healthy volunteers. Genotyping of both variants was done by the tetra-primer ARMS-PCR method, and the results were analyzed by the SPSS software package (version-25.0).
RESULTS: Logistic regression analysis indicated that in
case of IL-17A rs10484879 polymorphism, AC and AA genotype carriers showed 2.44- and 3.27-times significantly increased risk for CRC development (OR = 2.44, P = .0008 and OR = 3.27, P = .0133, individually). A significant association was also observed for AC + AA genotype (OR = 2.58, P = .0001). Again, over-dominant and allelic model revealed statistically significant link to CRC risk (OR = 2.13, P = .0035 and OR = 2.22, P = .001). For rs3748067 polymorphism, AG and AA genotype carriers showed 2.30- and 2.45-times enhanced risk for CRC (OR = 2.30, P = .005 and OR = 2.45, P = .031). A statistically significant association was also observed for AG + AA genotype (OR = 2.35, P = .001), over-dominant model (OR = 2.05, P = .014), and allelic model (OR = 2.11, P = .0004).
CONCLUSIONS: This study highlights that IL-17A rs10484879 and rs3748067 polymorphisms may be associated with CRC development. However, further functional research with larger samples may reveal more statistically significant outcomes.