非生物胁迫影响广泛的植物发育过程。其中,鉴于其对植物生长和形态发生的贡献,细胞扩增尤为重要。这里,我们描述了一个新的表型系统,以准确量化不同来源的非生物胁迫对细胞扩张能力的影响。这种方法监测拟南芥黄化幼苗的下胚轴生长,就像在黑暗中,这个胚胎器官仅仅通过扩增细胞来生长,没有细胞分裂。
Abiotic stress impacts a wide range of plant developmental processes. Among them, cell expansion is particularly important given its contribution to plant growth and morphogenesis. Here, we describe a new phenotypic system to quantify accurately the impact of different sources of abiotic stress on the cell\'s capacity to expand. This approach monitors hypocotyl growth in Arabidopsis thaliana etiolated seedlings, as in the dark this embryonic organ is known to grow solely by expanding its cells, without cell division.