苦荞麦是一种常见的功能性食品。它的谷物富含类黄酮和酚类。荞麦籽粒中黄酮类化合物和酚类物质的快速测定对促进荞麦产业的发展具有重要意义。这项研究,基于多重散射校正(MSC),标准化正态变量(SNV),倒数对数(Lg),一阶导数(FD),二阶导数(SD),和分数阶导数(FOD)预处理光谱,构建了苦荞麦籽粒总黄酮和总酚含量的高光谱监测模型。结果表明,SNV,Lg,FD,SD,和FOD预处理对原始光谱反射率有不同的影响,并且FOD还可以反映从原始光谱到整数阶导数光谱的变化过程。与原始光谱相比,MSC,SNV,Lg,FD,和SD变换光谱可以不同程度地提高光谱数据与总黄酮和总酚的相关性,不同目次的FOD谱与谷物中总黄酮和总酚的相关性不同。基于MSC的谷物中总黄酮和总酚的监测模型,SNV,Lg,FD,和SD变换光谱在SD和FD变换下取得了最好的精度,分别。因此,本研究进一步构建了基于FOD谱的谷物中总黄酮和总酚含量监测模型,并在1.6和0.6阶导数预处理下达到最佳精度,分别。R2c,RMSEc,R2v,RMSEv,总黄酮模型的RPD分别为0.8731、0.1332、0.8384、0.1448和2.4475,总酚模型为0.8296、0.2025、0.6535、0.1740和1.6713。该模型可实现苦荞麦籽粒中总黄酮和总酚含量的快速测定,分别。
Tartary buckwheat is a common functional food. Its grains are rich in flavonoids and phenols. The rapid measurement of flavonoids and phenols in buckwheat grains is of great significance in promoting the development of the buckwheat industry. This
study, based on multiple scattering correction (MSC), standardized normal variate (SNV), reciprocal logarithm (Lg), first-order derivative (FD), second-order derivative (SD), and fractional-order derivative (FOD) preprocessing spectra, constructed
hyperspectral monitoring models of total flavonoids content and total phenols content in tartary buckwheat grains. The results showed that SNV, Lg, FD, SD, and FOD preprocessing had different effects on the original spectral reflectance and that FOD can also reflect the change process from the original spectrum to the integer-order derivative spectrum. Compared with the original spectrum, MSC, SNV, Lg, FD, and SD transformation spectra can improve the correlation between spectral data and total flavonoids and total phenols in varying degrees, while the correlation between FOD spectra of different orders and total flavonoids and total phenols in grains was different. The monitoring models of total flavonoids and total phenols in grains based on MSC, SNV, Lg, FD, and SD transformation spectra achieved the best accuracy under SD and FD transformation, respectively. Therefore, this
study further constructed monitoring models of total flavonoids and total phenols content in grains based on the FOD spectrum and achieved the best accuracy under 1.6 and 0.6 order derivative preprocessing, respectively. The R2c, RMSEc, R2v, RMSEv, and RPD were 0.8731, 0.1332, 0.8384, 0.1448, and 2.4475 for the total flavonoids model, and 0.8296, 0.2025, 0.6535, 0.1740, and 1.6713 for the total phenols model. The model can realize the rapid measurement of total flavonoids content and total phenols content in tartary buckwheat grains, respectively.