归巢和雕刻，造血干细胞移植成功的决定因素定义为造血干/祖细胞(HSPCs)滞留受体骨髓的过程.SDF-1/CXCR4轴作为归位和雕刻的主要调节器，然而,CXCR4信号传导依赖于CXCR4及其配体SDF-1的表达,其是高度动态的。因此，本研究旨在探索CXCR4组成型活性突变体（CXCR4-CAM）在克服CXCR4信号传导限制并上调其归巢和植入效率方面的潜力。这些突变体的调节转基因表达研究表明，它们对内皮和细胞外基质蛋白的细胞粘附效率显着提高。这完全表明CXCR4-CAM在旨在提高HSPCs植入效率的研究中具有广阔的前景。
Homing and engraftment, a determining factor in hematopoietic stem cell transplantation success is defined as a process through which hematopoietic stem/progenitor cells (
HSPCs) lodge recipient bone marrow. SDF-1/CXCR4 axis acts as a principle regulator in homing and engraftment, however, CXCR4 signaling is dependent upon expression of CXCR4 and its ligand SDF-1, which is highly dynamic. Hence, present investigation was aimed to explore the potential of CXCR4 constitutive active mutants (CXCR4-CAMs) in overcoming the limitation of CXCR4 signaling and up-modulate its efficiency in homing and engraftment. Regulated transgene expression
study of these mutants revealed their significantly enhanced cell adhesion efficiency to endothelium and extracellular matrix protein. This altogether indicates promising prospects of CXCR4-CAMs in research aimed to improve
HSPCs engraftment efficiency.
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