目的:我们的研究旨在探讨短双歧杆菌207-1对特定神经递质和激素的功效以及调节健康成人生活方式行为的能力。
方法:总共,120名精神紧张的健康成年人,超重,失眠,便秘被随机分配接受低剂量的短芽孢杆菌207-1(LD,n=40),高剂量短芽孢杆菌207-1(HD,n=40),或安慰剂(n=40)28天。收集粪便和血液样本,并在试验前后回答问卷。使用酶联免疫吸附法检测神经递质和血清激素。使用16SrRNA测序评估肠道微生物群组成。短链脂肪酸(SCFA)浓度通过气相色谱-质谱(GC-MS)测定。
结果:我们研究的主要结果是心理健康的变化,包括神经递质,下丘脑-垂体-肾上腺(HPA)轴激素,和心理尺度。结果表明,在益生菌组中,γ-氨基丁酸(GABA)显着增加,HPA轴激素总体受到抑制,而5-羟色胺(5-HT)没有显着变化。然而,情绪量表评分无显著变化.次要结果集中在207-1调节健康成年人的身体和生活方式的能力(例如,睡眠,饮食,锻炼,等。).益生菌组的PSQI评分显著下降,改善睡眠质量。同时,益生菌组的运动摄入量略有增加,而膳食摄入量稳定.通过体检,参与者表现出体重减轻,但两组间无统计学显著性差异.然后,通过肠道微生物群验证,在207-1的有效干预下观察到肠道菌群的变化,而LD组的短链脂肪酸(SCFA)增加,特别是乙酸和丙酸。HD组的α多样性略有下降。
结论:短双歧杆菌207-1进入生物体,并通过微生物组-肠-脑轴影响神经递质和HPA轴激素水平。同时,207-1补充剂改善了健康成年人的日常生活行为,这又可能导致他们身体的变化(例如体重和脂质代谢)。然而,这项研究没有发现显著的情绪调节功效.整个研究的机制尚不清楚,但是我们假设SCFA可能是关键途径,未来需要更多的实验进行验证。
背景:该试验于2023年3月16日在中国临床试验注册中心进行了回顾性注册,登录号为ChiCTR2300069453。
OBJECTIVE: Our
study aimed to explore the efficacy of Bifidobacterium breve 207-1 on specific neurotransmitters and hormones and the ability to regulate lifestyle behaviors in healthy adults.
METHODS: In total, 120 healthy adults with high mental stress, overweight, insomnia, and constipation were randomly assigned to receive low-dose B. breve 207-1 (LD, n = 40), high-dose B. breve 207-1 (HD, n = 40), or placebo (n = 40) for 28 days. Fecal and blood samples were collected and questionnaires were answered before and after the
trial. Neurotransmitters and serum hormones were detected using enzyme-linked immunosorbent assay. The gut microbiota composition was assessed using 16 S rRNA sequencing. Short-chain fatty acids (SCFAs) concentrations were determined via gas chromatography-mass spectrometry (GC-MS).
RESULTS: The primary outcome of our study was changes in mental wellness, including neurotransmitters, the hypothalamic-pituitary-adrena (HPA) axis hormones, and the psychological scales. The results showed that γ-aminobutyric acid (GABA) increased significantly and the HPA axis hormones were suppressed overall in the probiotic groups while 5-hydroxytryptamine (5-HT) did not change significantly. However, there was no significant change in mood scale scores. The secondary outcome focused on the ability of 207-1 to regulate the body and lifestyle of healthy adults (e.g., sleep, diet, exercise, etc.). The PSQI scores in the probiotics groups significantly decreased, indicating improved sleep quality. Meanwhile, the probiotic groups had a slight increase in exercise consumption while dietary intake stabilized. By physical examination, the participants showed weight loss although no statistically significant difference was observed between the groups. Then, validated by gut microbiota, changes in the gut microbiota were observed under the effective intervention of 207-1 while short-chain fatty acids (SCFAs) increased in the LD group, particularly acetic and propionic acids. There was a slight decrease in alpha-diversity in the HD group.
CONCLUSIONS: Bifidobacterium breve 207-1 entered the organism and affected neurotransmitter and the HPA axis hormone levels via the microbiome-gut-brain axis. Meanwhile, 207-1 supplementation improved daily lifestyle behaviors in healthy adults, which may in turn lead to changes in their bodies (e.g. weight and lipid metabolism). However, this
study did not find significant mood-modulating efficacy. The mechanism of the overall
study is unclear, but we hypothesize that SCFAs may be the key pathway, and more experiments are needed for validation in the future.
BACKGROUND: This
trial was retrospectively registered in the Chinese Clinical
Trial Registry under the accession number ChiCTR2300069453 on March 16, 2023.