目的：优化西洋参的最佳提取工艺。
方法:(1)以人参皂苷Rg的含量和提取效率为指标,选择最佳的醇提法,Re,Rb,采用正交试验设计和综合多准则法。(2)采用RP-HPLC梯度洗脱法测定了西洋参中3种人参皂苷Rg1、Re和Rb1的含量。
结果：确定最佳的酒精提取程序为70％酒精回流12次，提取3h2次。
结论：最佳提取工艺可提高西洋参的提取效率。
OBJECTIVE: To optimize the best extracting procedure for Radix Panacis Quinquefolii.
METHODS: (1) The optimum alcohol extracting procedure was selected with the content and extraction efficiency of ginsenoside Rg, Re, Rb, from Radix Panacis Quinquefolii by orthogonal test design and synthesizing multiple
guidelines method. (2) The content of three ginsenoides Rg1, Re and Rb1 in Radix Panacis Quinquefolii was determined by gradient elution method in RP-HPLC.
RESULTS: The optimum alcohol-extracting procedure was determined as 12 times of 70% alcohol refluxing and extracting 3 h for 2 times.
CONCLUSIONS: The optimum extracting procedure can increase extraction efficiency of Radix Panacis Quinquefolii.
