目的:系统评价针刺治疗大鼠偏头痛实验模型的有效性和潜在机制。
方法:九个电子数据库,包括CNKI(中国国家知识基础设施),万方,VIP(中国科学期刊数据库),Sinomed,PubMed,科克伦图书馆,WebofScience和EBSCO,寻找涉及针刺干预的大鼠偏头痛的随机实验研究。搜索期从开始到2022年6月。使用SYRCLE的动物研究偏倚风险工具评估方法学质量。使用Revman5.3软件分析数据。
结果:本分析共纳入13项研究。从现有的实验研究中发现,针灸显着降低了偏头痛大鼠的行为评分(MD=-15.01,95CI=[-18.01,-12.01],P<0.00001)并下调降钙素基因相关肽(CGRP)的表达(MD=-16.14,95CI=[-21.45,-10.83],P<0.00001),P物质(SP)(MD=-11.47,95CI=[-15.97,-6.98],P<0.00001)和一氧化氮(NO)(MD=-3.02,95CI=[-3.79,-2.26],血清P<0.00001),和刺激性G蛋白(Gsa)(MD=-62.90,95CI=[-69.88,-55.92],脑干P<0.00001)。针刺还显着增加了抑制性G蛋白(Gia)的含量(MD=24.01,95CI=[20.10,27.92],P<0.00001)在脑干和50%缩爪阈值(50%PWT)(MD=1.96,95CI=[1.15,2.77],P<0.00001)。
结论:针刺能有效提高偏头痛患者的行为表现,其作用机制可能涉及抑制脑膜血管舒张和炎症因子,和减少神经源性炎症。
To systematically evaluate the effectiveness and potential underlying mechanisms of acupuncture in the treatment of experimental model of migraine in rats.
Nine electronic databases, including CNKI (China National Knowledge Infrastructure), WanFang, VIP (Chinese Scientific Journals Database), Sinomed, PubMed, Cochrane Library, Web of Science and EBSCO, were searched for randomized experimental studies on migraine in rats involving acupuncture intervention. The search period ranged from inception to June 2022. The methodological quality was assessed using the SYRCLE\'s risk of bias tool for animal studies. Data were analyzed using the Revman 5.3 software.
A total of 13 studies were included in this analysis. Findings from the available experimental studies documented that acupuncture significantly reduced behavior scores of rats with migraine (MD = -15.01, 95%CI = [-18.01, -12.01], P<0.00001) and downregulated the expression of calcitonin gene-related peptide (CGRP) (MD = -16.14, 95%CI = [-21.45, -10.83], P<0.00001), substance P (SP) (MD = -11.47, 95%CI = [-15.97, -6.98], P<0.00001) and nitric oxide (NO) (MD = -3.02, 95%CI = [-3.79, -2.26], P<0.00001) in serum, and stimulatory G protein (Gsa) (MD = -62.90, 95%CI = [-69.88, -55.92], P<0.00001) in brainstem. Acupuncture also significantly increased the content of inhibitory G protein (Gia) (MD = 24.01, 95%CI = [20.10, 27.92], P<0.00001) in brainstem and 50% paw withdrawal threshold (50%PWT) (MD = 1.96, 95%CI = [1.15, 2.77], P<0.00001).
Acupuncture can effectively improve the behavioral performance of rates with migraine, and its mechanism of action might involve the inhibition of meningeal vasodilation and inflammatory factors, and the reduction of neurogenic inflammation.