背景:透明相关形式1(DIAPH1)是晚期糖基化终产物(AGE)信号转导的一种新成分,最近发现其参与糖尿病相关疾病,肥胖,和雄激素激素.我们调查了血浆DIAPH1水平是否是多囊卵巢综合征(PCOS)的潜在预后预测因子。
方法:循环血浆DIAPH1水平和葡萄糖指标,胰岛素,脂质代谢,肝酶,肾功能,性激素,对75例PCOS患者和77例健康参与者进行了炎症检测。将所有参与者分为正常体重(NW)和超重/肥胖(OW)亚组。用R工作室进行统计分析。
结果:PCOS患者表现为高雄激素血症,促黄体生成素/促卵泡激素(LH/FSH)增加,体内脂肪和胰岛素抵抗.与对照组相比,PCOS女性的血浆DIAPH1水平显着降低,与OW对照组相比,OWPCOS患者的DIAPH1水平明显降低(P<0.001)。DIAPH1水平与空腹血糖(FBG)相关,总胆固醇(TC),β细胞功能的稳态模型评估(HOMA-β),所有参与者的LH/FSH(FBG:r=0.351,P<0.0001;TC:r=0.178,P=0.029;HOMA-β:r=-0.211,P=0.009;LH/FSH:r=-0.172,P=0.040)。多因素logistic回归分析显示血浆DIAPH1水平是PCOS的独立危险因素。包含DIAPH1,BMI,FBG,睾酮被用来预测PCOS的风险,敏感性为92.0%,特异性为80.9%。建立列线图以促进临床诊断。
结论:这些发现提示血浆DIAPH1与糖代谢有关,胰岛素抵抗,和性激素和支持DIAPH1作为PCOS的潜在预测因素。
BACKGROUND: Diaphanous related formin 1 (DIAPH1) is a novel component of advanced glycation end product (AGE) signal transduction that was recently found to participate in diabetes-related disorders, obesity, and androgen hormones. We investigated whether plasma DIAPH1 levels were a potential prognostic predictor for polycystic ovary syndrome (PCOS).
METHODS: The levels of circulating plasma DIAPH1 and indicators of glucose, insulin, lipid metabolism, liver enzymes, kidney function, sex hormones, and inflammation were measured in 75 patients with PCOS and 77 healthy participants. All of the participants were divided into normal-weight (NW) and overweight/obese (OW) subgroups. Statistical analyses were performed with R studio.
RESULTS: PCOS patients manifested hyperandrogenism, increased luteinizing hormone/follicle-stimulating hormone (LH/FSH), and accumulated body fat and insulin resistance. Plasma DIAPH1 levels were significantly decreased in women with PCOS compared to control participants, and DIAPH1 levels were distinctly reduced in OW PCOS compared to OW control subjects (P < 0.001). DIAPH1 levels correlated with fasting blood glucose (FBG), total cholesterol (TC), the homeostasis model assessment of β-cell function (HOMA-β), and LH/FSH in all participants (FBG: r = 0.351, P < 0.0001; TC: r = 0.178, P = 0.029; HOMA-β: r = -0.211, P = 0.009; LH/FSH: r = -0.172, P = 0.040). Multivariate logistic regression analysis revealed that plasma DIAPH1 levels were an independent risk factor for PCOS. A model containing DIAPH1, BMI, FBG, and testosterone was constructed to predict the risk of PCOS, with a sensitivity of 92.0% and a specificity of 80.9%. A nomogram was constructed to facilitate clinical diagnosis.
CONCLUSIONS: These findings suggest the association of plasma DIAPH1 with glucose metabolism, insulin resistance, and sex hormones and support DIAPH1 as a potential predictive factor for PCOS.