肠道病毒D68(EV-D68),作为儿科呼吸道疾病的主要病原体之一,近年来在人群中广泛传播。作为病毒抗原性的基础,抗原表位是监测病毒抗原性转化的关键。然而,缺乏对EV-D68抗原表位的系统研究。在这项研究中,基于生物信息学的人类肠道病毒预测算法用于预测EV-D68的构象表位.预测结果表明,EV-D68的构象表位聚集在三个位点:位点1,位点2和位点3。场地1位于五重轴附近的峡谷“北缘”区域;场地2位于两重轴附近的“泡芙”区域;场地3由两部分组成,一个在峡谷南缘的“旋钮”区域,另一个在三重轴区域。预测的表位与四种报道的单克隆抗体(mAb)的结合区域高度重叠,这表明预测是高度可靠的。系统发育分析表明,VP1BC环表位的氨基酸突变,DE回路,C端,VP2EF环在EV-D68进化枝/子进化枝和流行病的进化分化中起着至关重要的作用。这一发现表明VP1BC循环,DE回路,C端,和VP2的EF环是EV-D68最重要的表位。对EV-D68表位的研究将有助于疫情监测以及诊断试剂和重组疫苗的开发。
Enterovirus D68 (EV-D68), as one of the major pathogens of paediatric respiratory disease, has been widely spread in the population in recent years. As the basis of virus antigenicity, antigenic epitopes are essential to monitoring the transformation of virus antigenicity. However, there is a lack of systematic studies on the antigenic epitopes of EV-D68. In this
study, a bioinformatics-based prediction algorithm for human enteroviruses was used to predict the conformational epitopes of EV-D68. The prediction results showed that the conformational epitopes of EV-D68 were clustered into three sites: site 1, site 2, and site 3. Site 1 was located in the \"north rim\" region of the canyon near the fivefold axis; site 2 was located in the \"puff\" region near the twofold axis; and site 3 consisted of two parts, one in the \"knob\" region on the south rim of the canyon and the other in the threefold axis region. The predicted epitopes overlapped highly with the binding regions of four reported monoclonal antibodies (mAbs), indicating that the predictions were highly reliable. Phylogenetic analysis showed that amino acid mutations in the epitopes of the VP1 BC loop, DE loop, C-terminus, and VP2 EF loop played a crucial role in the evolutionary divergence of EV-D68 clades/subclades and epidemics. This finding indicated that the VP1 BC loop, DE loop, C-terminus, and VP2 EF loop were the most important epitopes of EV-D68. Research on the epitopes of EV-D68 will contribute to outbreak surveillance and to the development of diagnostic reagents and recombinant vaccines.