微孢子虫是一组具有人畜共患潜力的孢子形成微生物。这项研究旨在比较猫主人和非宠物主人的肠道微孢子虫感染。总的来说,从室内猫收集了210份粪便样本,猫的主人,和非宠物主人。进行DNA提取并扩增小亚基核糖体RNA(SSUrRNA)基因。为了表征基因型,对内部转录间隔区(ITS)片段进行扩增和测序。绘制了系统发育树,以评估肠孢子虫分离株之间的关系。来自猫主人的两个(2.9%)和一个(1.4%)粪便样本以及来自非宠物主人的一个(1.4%)和两个(2.9%)粪便样本对E.bieneusi和头孢菌素肠呈阳性,分别。在两个猫样品中检测到E.bieneusi(2.9%)。在被感染的猫和它们的主人之间没有看到相同的感染。猫主人和非宠物主人的微孢子虫患病率之间没有显着差异。的确,基因型L型和IV型在猫中可见,而基因型D仅在人类中检测到。在这项研究中,E.bieneusi和E.inteutinalis在猫主人和非宠物主人中更为普遍,分别。的确,E.bieneusi在猫及其主人中的患病率较高可能是由于该物种在全球范围内的分布。
Microsporidia is a group of spore-forming microorganisms with zoonotic potential. This study aimed to compare intestinal microsporidia infections in cat owners and non-pet owners. In total, 210 fecal samples were collected from indoor cats, cat owners, and non-pet owners. DNA extraction was performed and the small subunit ribosomal RNA (SSU rRNA) gene was amplified. To characterize the genotypes, the internal transcribed spacer (ITS) fragment was amplified and sequenced. The phylogenetic trees were drawn to evaluate the relationship among Enterocytozoon bieneusi isolates. Two (2.9%) and one (1.4%) fecal samples from cat owners and one (1.4%) and two (2.9%) fecal samples from non-pet owners were positive for E. bieneusi and
Encephalitozoon intestinalis, respectively. E. bieneusi was detected in two cat samples (2.9%). Same infection was not seen between infected cats and their owners. There was no significant difference between the prevalence rate of microsporidia among the cat owners and non-pet owners. Indeed, the genotypes L and type IV were seen in cats, while the genotype D was only detected in human. In this study, E. bieneusi and E. intestinalis were more prevalent among the cat owners and non-pet owners, respectively. Indeed, the higher prevalence of E. bieneusi in cats and their owners might be resulted from the worldwide distribution of this species.