高效液相色谱(HPLC)是分离和测定具有复杂性质的新药的最有用的技术之一。HPLC检测器的选择取决于分子的化学性质,潜在的杂质,样本的矩阵,灵敏度,可用性,和/或检测器的成本。使用UV/Vis检测器的HPLC方法是制药应用中最常用且简单的分析程序,但它仅限于具有发色团的化合物。因此,这篇综述概述了弱发色团化合物分析方法的发展。这篇综述描述了PubMed中有关基于HPLC的方法的精选论文,Scopus,语义学者和科学直接数据库,基本上在2006年至2023年之间。在分析研究中,UV-Vis的HPLC方法,FLD,CAD,ELSD,RID,ECD,发现了CLND和MS检测。这项研究是对科学文献中描述的不同类型的检测的比较,通常用于具有弱发色团的化合物。预计本综述将有助于分析开发领域的科学家改进与候选药物相关的研究,并根据监管水平确保其质量。
High-performance liquid chromatography (HPLC) is one of the most useful techniques for the separation and determination of new drugs with a complex nature. The selection of an HPLC
detector depends on the chemical nature of molecules, potential impurities, matrix of the sample, sensitivity, availability, and/or cost of the
detector. HPLC methods with UV/Vis detectors are the most used and simple analytical procedures in pharmaceutical applications, but it is limited to compounds that possess a chromophore. Hence, this
review provides an overview on the development of analytical methods for compounds with weak chromophores. The
review described selected papers about HPLC based methods in the PubMed, Scopus, Semantic Scholar and ScienceDirect databases, basically between 2006 and 2023. Of the analytical studies, the HPLC methods with UV-Vis, FLD, CAD, ELSD, RID, ECD, CLND and MS detection were found. This study is a comparison of different types of detection that are described in scientific literature and are routinely used for compounds with weak chromophores. It is expected that this
review will be helpful for scientists in the analytical development fields to improve research related to the drug candidates and to ensure its quality according to regulatory levels.