幽门螺杆菌感染是人类最常见的感染性疾病之一。牙菌斑被认为是这种细菌的储库,这可能在胃肠道问题的发展中起重要作用。我们的目的是调查有和没有龋齿的儿童牙菌斑中幽门螺杆菌的患病率及其毒力因子。
在6至12岁的儿童中,共有72名儿童参加了这项研究,包括36例总DMFT/dmft>3(病例组)和36例总DMFT/dmft<1(对照组)。在从下第一恒磨牙中去除牙龈上牙后,使用PCR方法检查样本中是否存在幽门螺杆菌及其一些毒力因子.使用卡方进行统计分析,费希尔精确检验,t检验,和逻辑回归。
在72名参与者中,40例男性,女性32例。总DMFT/dmft指数的最小值和最大值分别为零和十,分别,总DMFT/dmft的平均值±SD值为2.78±3.22。除蔬菜消费外(p=0.045),两组在胃肠道疾病方面没有显着差异,婴儿期喂养方法(p=0.058),每日刷牙频率(p=0.808),牙科就诊频率(p=0.101),以及牙科刮伤史(p=0.246)和专业的局部氟化物治疗(p=0.5)。在72个样本中,HpDNA阳性15例(20.8%),牙菌斑中这种细菌的存在与龋齿之间没有显着关联(p=0.281)。15例幽门螺杆菌中检测到的毒力因子频率如下:6例cagA(40.0%),vacAm1在三种情况下(20.0%),和vacAs1在一起(6.7%)。在毒力因子的患病率方面,两组之间没有显着差异。
我们的结果表明,幽门螺杆菌和一些毒力因子在牙菌斑中的存在是伊朗儿童中这种细菌的储库。尽管该细菌与龋齿的发生率之间没有显着关联,需要认真考虑儿童的牙齿健康。
Helicobacter pylori infection is one of the most common infectious diseases in humans. Dental plaque is considered as a reservoir of this bacterium, which could play an important role in the development of gastrointestinal problems. Our aim was to investigate the prevalence of H. pylori and its virulence factors in dental plaques in children with and without dental caries.
Among children aged 6 to 12 years, a total of 72 children were enrolled in the study, including 36 cases with total DMFT/dmft > 3 (
case group) and 36 participants with total DMFT/dmft < 1 (control group). After removing supra-gingival plaques from the lower first permanent molar teeth, the samples were examined using PCR method for the presence of H. pylori and some of its virulence factors. Statistical analysis was performed using chi-square, Fisher\' exact test, t-tests, and logistic regression.
Of 72 participants, 40 cases were male, and 32 cases were female. The minimum and maximum values of total DMFT/dmft indices were zero and ten, respectively, and the mean ± SD value of total DMFT/dmft was 2.78 ± 3.22. Except for vegetable consumption (p = 0.045), there was no significant difference between the two groups regarding gastrointestinal disorders, feeding methods in infancy (p = 0.058), frequency of daily brushing (p = 0.808), frequency of dental visits (p = 0.101), and history of dental scaling (p = 0.246) and professional topical fluoride therapy (p = 0.5). Out of 72 samples, 15 cases were positive for H. pylori DNA (20.8%), and there was no significant association between the presence of this bacterium in dental plaque and dental caries (p = 0.281). The frequency of virulence factors detected in 15 H. pylori cases was as follows: cagA in six cases (40.0%), vacAm1 in three cases (20.0%), and vacAs1 in one
case (6.7%). There was no significant difference between the groups regarding the prevalence of virulence factors.
Our results indicate the presence of H. pylori along with some virulence factors in dental plaques as a reservoir of this bacterium in children in Iran. Although there was no significant association between this bacterium and the incidence of dental caries, dental health in children needs to be seriously taken into consideration.