背景:温度是硬骨鱼活力和发育的关键环境决定因素,然而,他们感知温度波动的潜在机制在很大程度上仍未被探索。瞬时受体电位(TRP)蛋白,以参与温度传感而闻名,没有被描述为硬骨鱼,特别是关于他们的温度传感能力。
结果:在这项研究中,进行了全基因组分析,鉴定了普通话Sinipercachuatsi中的28个TRP基因。这些基因被归类为TRPA家族,TRPC,TRPP,TRPM,TRPML,和TRPV。尽管不同亚科的保守基序存在显著差异,TRP家族成员具有共同的结构特征,包括锚蛋白重复序列和TRP结构域。组织表达分析显示这些TRP基因中的每一个都表现出独特的表达模式。此外,在暴露于高温和低温胁迫后,对10个选定的TRP基因的组织表达模式进行检查表明,TRP基因的表达对温度变化有反应。此外,在Sinipercachuatsi弹状病毒感染后,TRP基因的表达谱显示大多数基因显着上调,橘鱼虹彩病毒和传染性脾肾坏死病毒感染。
结论:这项研究在整个基因组范围内表征了普通话鱼的TRP家族基因,并探索了它们对温度应激和病毒感染的反应模式。我们的工作将增进对鱼类TRP渠道及其可能功能的整体了解。
BACKGROUND: Temperature is a crucial environmental determinant for the vitality and development of teleost fish, yet the underlying mechanisms by which they sense temperature fluctuations remain largely unexplored. Transient receptor potential (TRP) proteins, renowned for their involvement in temperature sensing, have not been characterized in teleost fish, especially regarding their temperature-sensing capabilities.
RESULTS: In this study, a genome-wide analysis was conducted, identifying a total of 28 TRP genes in the mandarin fish Siniperca chuatsi. These genes were categorized into the families of TRPA, TRPC, TRPP, TRPM, TRPML, and TRPV. Despite notable variations in conserved motifs across different subfamilies, TRP family members shared common structural features, including ankyrin repeats and the TRP domain. Tissue expression analysis showed that each of these TRP genes exhibited a unique expression pattern. Furthermore, examination of the tissue expression patterns of ten selected TRP genes following exposure to both high and low temperature stress indicated the expression of TRP genes were responsive to temperatures changes. Moreover, the expression profiles of TRP genes in response to mandarin fish virus infections showed significant upregulation for most genes after Siniperca chuatsi rhabdovirus, mandarin fish iridovirus and infectious spleen and kidney necrosis virus infection.
CONCLUSIONS: This study characterized the TRP family genes in mandarin fish genome-wide, and explored their expression patterns in response to temperature stress and virus infections. Our work will enhance the overall understanding of fish TRP channels and their possible functions.