PDF(Pubmed)
Abstract：
Pneumoconiosis is a common occupational disease that can worsen with accompanying infection. Torque teno virus (TTV) is a prevalent human virus with multiple genotypes that can chronically and persistently infect individuals. However, the prevalence of TTV in pneumoconiosis patients is still unclear. This research aims to detect the presence and prevalence of TTV in the alveolar lavage fluid of pneumoconiosis patients in the Hunan Province of China using PCR. As a result, a 65.5% positive rate (19 out of 29) of TTV was detected. The TTV detection rate varies among different stages of silicosis and different pneumoconiosis patient ages. Nine novel TTV genomes ranging in size from 3719 to 3908 nt, named TTV HNPP1, HNPP2, HNPP3, HNPP4, HNPP5, HNPP6-1, HNPP6-2, HNPP7-1 and HNPP7-2, were identified. A genomic comparison and phylogenetic analysis indicated that these nine TTVs represent five different species with high genetic diversity which belong to the genus Alphatorquevirus. HNPP6-1 and HNPP6-2 belong to TTV3, HNPP5 belongs to TTV13, HNPP1 belongs to TTV24, HNPP4 belongs to TTV20, and the others belong to TTV19. The genomes of TTV HNPP1, HNPP6-1, and HNPP6-2 contain three putative open reading frames (ORFs) coding for proteins, ORF1, ORF2, and ORF3, while the other six TTV genomes contain two ORFs coding for proteins, ORF1 and ORF2. These results provide the first description of TTV epidemiology in pneumoconiosis patients in China. The newly identified TTV genome sequences reveal the high genetic diversity of TTV in pneumoconiosis patients and could contribute to a deeper understanding of TTV retention and infection in humans.
摘要：
尘肺是一种常见的职业病，可伴随感染加重。扭矩特诺病毒（TTV）是一种流行的人类病毒，具有多种基因型，可以长期和持续地感染个体。然而,尘肺患者中TTV的患病率尚不清楚.本研究旨在使用PCR检测中国湖南省尘肺患者肺泡灌洗液中TTV的存在和流行情况。因此,检测到65.5％的TTV阳性率（29个中的19个）。TTV检出率在矽肺的不同阶段和不同尘肺患者年龄之间存在差异。九个新的TTV基因组，大小从3719到3908nt，鉴定了命名为TTV的HNPP1、HNPP2、HNPP3、HNPP4、HNPP5、HNPP6-1、HNPP6-2、HNPP7-1和HNPP7-2。基因组比较和系统发育分析表明，这9个TTV代表五个具有高遗传多样性的不同物种，属于Alphatorquesus属。HNPP6-1和HNPP6-2属于TTV3,HNPP5属于TTV13,HNPP1属于TTV24,HNPP4属于TTV20,其余属于TTV19。TTVHNPP1，HNPP6-1和HNPP6-2的基因组包含三个假定的编码蛋白质的开放阅读框（ORF），ORF1，ORF2和ORF3，而其他六个TTV基因组包含两个编码蛋白质的ORFs，ORF1和ORF2。这些结果首次描述了中国尘肺患者的TTV流行病学。新鉴定的TTV基因组序列揭示了尘肺患者TTV的高度遗传多样性，并可能有助于更深入地了解人类的TTV保留和感染。
