背景:小分子荧光探针在不同宫颈病变中甲基化检测的价值分析。
方法:(1)4组不同病变组织的灰度值差异显著(p<0.05)。两组之间灰度值的比较显示,CA组明显超过LSIL和宫颈炎组,且HSIL组明显高于LSIL组和宫颈炎组(p<0.05);(2)以55.21为中线计算入组受试者的平均灰度值,>55.21为正,≤55.21为负。
结果:结果显示,宫颈炎组的阳性率为0.00%,LSIL组67.74%,HSIL组83.33%,和CA组100.00%。四组之间的结果明显不同(p<0.05);(3)DAPI之间的比较,探针,明亮,和合并的宫颈炎图像,LSIL,HSIL,和CA表明不同的宫颈病变有相当不同的染色。
结论:灰度值,阳性率,不同宫颈病变的染色图像明显不同。该小分子荧光探针对宫颈病变具有较好的鉴别价值,可考虑推广应用。
BACKGROUND: Value analysis of a small-molecule fluorescent probe for methylation detection in different cervical lesions.
METHODS: (1) The grayscale values of distinct lesion tissues were remarkably distinct among the four groups (p < 0.05). The comparison of the grayscale value between the two groups showed that the CA group noticeably exceeded the LSIL and cervicitis groups, and the HSIL group was apparently higher than the LSIL and cervicitis groups (p < 0.05); (2) The mean grayscale values of the enrolled subjects were calculated with 55.21 as the midline, with >55.21 as positive and ≤55.21 as negative.
RESULTS: The results showed that the positive rate of the cervicitis group was 0.00%, the LSIL group 67.74%, the HSIL group 83.33%, and the CA group 100.00%. The results among the four groups were notably distinct (p < 0.05); (3) The comparison among DAPI, probe, bright, and merged images of cervicitis, LSIL, HSIL, and CA indicated that different cervical lesions were with quite various stains.
CONCLUSIONS: The grayscale value, positive rate, and stained picture of distinct cervical lesions were remarkably different. The small-molecule fluorescent probe has a good value in differentiating cervical lesions and can be considered for popularization and application.