提出了全球朝鲜蓟的综合共识连锁图。母系和父系遗传图谱是根据来自朝鲜蓟基因型(Mola)与其祖先杂交的F(1)后代构建的,野生卡顿(Tolfa),使用EST派生的SSR,基因组SSRs,AFLP,十个基因,和两个形态特征。对于大多数基因来说,主要属于绿原酸途径,开发新的标记。其中五个是通过高分辨率熔解技术分析的SNP标记。从母亲(Mola)和父亲(Tolfa)地图中,获得了一张综合地图,包含337个分子和一个按17个连锁群(LGs)排序的形态标记,Mola和Tolfa之间有联系.综合地图涵盖1,488.8cM,标记之间的平均距离为4.4cM。该地图与朝鲜蓟现有的地图对齐,和12个LGs通过31个桥标记连接。已经提出了LG编号。共有124个EST-SSRs和两个基因首次在这里定位,为朝鲜蓟功能图的构建提供了一个框架。建立共识图代表了计划全球朝鲜蓟基因组完整测序的必要条件。
An integrated
consensus linkage map is proposed for globe artichoke. Maternal and paternal genetic maps were constructed on the basis of an F(1) progeny derived from crossing an artichoke genotype (Mola) with its progenitor, the wild cardoon (Tolfa), using EST-derived SSRs, genomic SSRs, AFLPs, ten genes, and two morphological traits. For most genes, mainly belonging to the chlorogenic acid pathway, new markers were developed. Five of these were SNP markers analyzed through high-resolution melt technology. From the maternal (Mola) and paternal (Tolfa) maps, an integrated map was obtained, containing 337 molecular and one morphological markers ordered in 17 linkage groups (LGs), linked between Mola and Tolfa. The integrated map covers 1,488.8 cM, with an average distance of 4.4 cM between markers. The map was aligned with already existing maps for artichoke, and 12 LGs were linked via 31 bridge markers. LG numbering has been proposed. A total of 124 EST-SSRs and two genes were mapped here for the first time, providing a framework for the construction of a functional map in artichoke. The establishment of a
consensus map represents a necessary condition to plan a complete sequencing of the globe artichoke genome.