细胞周期蛋白依赖性激酶(CDK)控制细胞周期的进展。D型细胞周期蛋白(CYCD)通常被认为与CDK形成复合物并控制G1/S转变。在植物中,CYCD和CDK基因家族可分为6个(D1-D7)和7个(CDKA-CDKG)亚类,分别。CYCD和CDK家族中的不同亚类具有不同的数量,结构和功能。在一些异源木本植物中,这些亚类家族成员的功能尚不清楚.在这项研究中,在异二倍体毛白杨中鉴定出43个CYCD和27个CDK基因家族成员。系统发育分析表明,这些CYCD和CDK分为6个和7个亚类,分别,与其他物种相同。蛋白质性质的分析,基因结构,图案,域,这些CYCD和CDK的所有成员的顺式作用元件和组织特异性表达表明,不同亚类成员之间的差异很大,但同一亚类的成员,尤其是CDK基因家族的成员非常相似。这些发现还表明CYCD和CDK基因家族成员在响应激素和特异性表达方面具有很强的相关性。毛白杨的共线分析,毛果杨和拟南芥表明CYCD和CDK基因家族的扩增模式主要是全基因组重复(WGD)。CYCD和CDKs不同亚类的蛋白质相互作用预测结果表明,CYCD和CDKs不同亚类之间的相互作用存在显著差异。我们先前的研究发现,转基因PtoCYCD2;1和PtoCYCD3;3杨树表现出相反的表型。Y2H和BIFC成果显示,PtoCYCD2;1与PtoCYCD3;3与CDKs之间的互相感化差别明显。这一发现可能表明,不同CYCD亚类在植物生长发育中的功能差异与CYCD和CDK之间的不同相互作用密切相关。本研究结果为木本植物CYCD和CDK蛋白的功能研究提供了良好的思路和方向。
Cyclin-dependent kinases (CDKs) control the progression of the cell cycle. D-type cyclin (CYCD) is generally believed to form a complex with CDK and control the G1/S transition. In plants, CYCD and CDK gene families can be divided into 6 (D1-D7) and 7 (CDKA-CDKG) subclasses, respectively. Different subclasses in the CYCD and CDK families have different numbers, structures and functions. In some heterologous woody plants, the functions of these subclass family members remain unclear. In this
study, 43 CYCD and 27 CDK gene family members were identified in the allodiploid Populus tomentosa Carr. Phylogenetic analysis suggested that these CYCDs and CDKs were divided into 6 and 7 subclasses, respectively, which were the same as other species. The analysis of protein properties, gene structure, motifs, domains, cis-acting elements and tissue-specific expression of all members of these CYCDs and CDKs showed that the differences between members of different subclasses varied widely, but members of the same subclass especially in the CDK gene family were very similar. These findings also demonstrated a strong correlation between CYCD and CDK gene family members in response to hormones and specific expression. The collinear analysis of P. tomentosa, Populus trichocarpa and Arabidopsis thaliana showed that the expansion patterns of CYCD and CDK gene families were predominantly whole genome duplications (WGD). The protein interaction prediction results of different subclasses of CYCD and CDKs showed that the interaction between different subclasses of CYCD and CDKs was significantly different. Our previous
study found that transgenic PtoCYCD2;1 and PtoCYCD3;3 poplars exhibited opposite phenotypes. Y2H and BIFC results showed that the interaction between PtoCYCD2;1 and PtoCYCD3;3 was significantly different with CDKs. This finding might suggest that the functional differences of different CYCD subclasses in plant growth and development were closely related to the different interactions between CYCD and CDK. Our results provide a good idea and direction for the functional
study of CYCD and CDK proteins in woody plants.