背景:精液冷冻保存是一种广泛使用的生育力保存程序,尽管解冻后精子可能会发生一定程度的冷冻损伤。然而,有证据表明乳酸杆菌,在精液中发现的一种细菌,可能有利于精子质量。
目的:本研究旨在确定是否添加植物乳杆菌(L。plantarum)精子冷冻培养基的分泌物对精子运动有影响,形态学,和DNA片段化。
方法:这是一项前瞻性自动对照研究。这项研究是对来自生育中心精液分析的30名不育男性的30个原始精液样本进行的。在冻结之前,所有样品都进行了运动性分析,形态学,和DNA碎片百分比。然后将每个样品平均分成三个等分试样。使用以下三种培养基之一对每个等分试样进行冷冻保存:无植物乳杆菌分泌物(对照组),107CFU/ml,或108CFU/ml植物乳杆菌分泌物。精子运动,形态学,在添加冷冻保存培养基和精液解冻后评估DNA完整性。
结果:这项研究的结果表明,解冻后,与新鲜生精液相比,在补充有108CFU/ml植物乳杆菌分泌物的精子冷冻培养基中,未检测到进行性运动和非进行性百分比的统计学显着降低。此外,多元线性回归模型分析表明,渐进运动(p=0.02),非进行性运动性(p=0.016),与新鲜生精液相比,冷冻培养基(无植物乳杆菌分泌物)中的非活动精子(p=0.012)百分比显着降低。
结论:据我们所知,这是第一项研究表明,当添加到精子冷冻培养基中时,植物乳杆菌分泌物对精子运动具有冷冻保护作用。此外,在非正常精子症组中,发现植物乳杆菌分泌物比无植物乳杆菌分泌物的冷冻培养基更有效地保护精子DNA完整性。因此,必须优化冷冻保存程序,以最大程度地减少任何医源性诱导的精子DNA损伤。考虑到精子DNA损伤和子代突变负荷增加之间的相关性。本文受版权保护。保留所有权利。
Semen cryopreservation is a widely used procedure for fertility preservation, despite some level of cryodamage that may occur in spermatozoa after thawing. However, there is some evidence that lactobacilli, one of the bacteria found in semen, might benefit sperm quality.
This
study aims to determine whether the addition of Lactobacillus plantarum secretions to sperm freezing medium has an impact on sperm motility, morphology, and DNA fragmentation.
This is a prospective auto-controlled
study. It was conducted on 30 raw semen samples from 30 infertile men attending a fertility center for semen analysis. Before freezing, all the samples were analyzed for motility, morphology, and DNA fragmentation percentages. Each sample was then divided equally into three aliquots. Cryopreservation was performed on each aliquot using one of the following three media: without Lactobacillus plantarum secretions (control group) or with 107 or 108 colony-forming units/mL Lactobacillus plantarum secretions. Sperm motility, morphology, and DNA integrity were evaluated after the cryopreservation media were added and after semen thawing.
The results of this
study indicated that after thawing, no statistically significant decrease in progressive motility and non-progressive percentages were detected in the sperm freezing medium supplemented with 108 colony-forming units/mL Lactobacillus plantarum secretions than the fresh raw semen. Moreover, multivariate linear regression model analyses showed that the progressive motility (p = 0.02), non-progressive motility (p = 0.016), and non-motile spermatozoa (p = 0.012) percentages were significantly decreased in the freezing medium (without Lactobacillus plantarum secretions) compared to the fresh raw semen.
To the best of our knowledge, this is the first
study showing that Lactobacillus plantarum secretions had a cryoprotective effect on sperm motility when added to the sperm freezing medium. Furthermore, Lactobacillus plantarum secretions were found to protect sperm DNA integrity more effectively than the freezing medium without Lactobacillus plantarum secretions in non-normozoospermia group. Cryopreservation procedures must therefore be optimized to minimize any iatrogenically induced sperm DNA damage, given the correlation between sperm DNA damage and increased mutation loads in progeny.