RESULTS: All PRP concentrations in both extenders, particularly 10% PRP, improved the quality and functionality of the sperm in both equilibrated and frozen-thawed semen. Additionally, the antioxidant enzyme activities in both extenders were higher in the PRP-supplemented groups compared to the control group in thawed semen (P < 0.05). All post-thaw sperm quality, antioxidant enzyme activities, and functionality aside from DNA integrity were higher (P < 0.05) in the PRP-supplemented OptiXcell® than in the PRP-supplemented Tris egg yolk-based extender. The fertility of cryopreserved semen in the extenders supplemented with 10% and 15% PRP increased (P < 0.05) significantly more than that of the control extenders, with 10% PRP being the optimum concentration in OptiXcell® (80%) compared to that of Tris egg yolk-based extender (66.67%) and control of two extenders (53.33% and 46.67%, respectively).
CONCLUSIONS: Even though autologous PRP-supplemented extenders have a protective impact on equilibrated and cryopreserved semen, 10% PRP-supplemented OptiXcell® extenders are more effective at preserving post-thaw semen quality, functionality, and antioxidant capacity, which increases the in vivo fertility of buffalo bulls.
结果:两种增量剂中的所有PRP浓度,特别是10%的PRP,提高了平衡精液和冻融精液中精子的质量和功能。此外,与解冻精液中的对照组相比,PRP补充组的两种补充剂中的抗氧化酶活性均较高(P<0.05)。所有解冻后的精子质量,抗氧化酶活性,补充PRP的OptiXcell®比补充PRP的基于Tris蛋黄的延伸剂中除了DNA完整性之外的功能性更高(P<0.05)。补充10%和15%PRP的补充剂中冷冻保存的精液的生育力显着增加(P<0.05)比对照补充剂的生育力增加(P<0.05)。与基于Tris蛋黄的补充剂(66.67%)和两种补充剂的对照(53.33%和46.67%)相比,OptiXcell®中10%PRP是最佳浓度(80%),分别)。
结论:尽管补充了自体PRP的补充剂对平衡和冷冻保存的精液有保护作用,10%PRP补充的OptiXcell®补充剂在保存解冻后精液质量方面更有效,功能,和抗氧化能力,这增加了水牛公牛的体内生育能力。