PDF(Pubmed)
Abstract：
BACKGROUND: Buffalo spermatozoa have a distinct membrane structure that makes them more vulnerable to cryopreservation, resulting in lower-quality post-thawed sperm. This decreases the success rate of artificial insemination in buffaloes. Understanding and addressing these specific vulnerabilities are essential for improving reproductive techniques in buffalo populations. The properties of cryopreserved buffalo bull semen were examined in this study regarding the impact of adding autologous platelet-rich plasma (PRP) to OptiXcell® or Tris egg yolk-based extenders. Ten buffalo bulls were used to collect semen. Each bull\'s ejaculate was separated into two main equal amounts, each of which was then diluted with either OptiXcell® or Tris egg yolk-based extender, supplemented with various PRP concentrations (5%, 10%, and 15%), and the control (0%), before being cryopreserved according to established protocols. Following equilibration and thawing, the quality and functionality of the sperm were evaluated, along with the antioxidant enzyme activities (GSH and TAC), malondialdehyde (MDA) content, and in vivo fertilization rate of the thawed semen.
RESULTS: All PRP concentrations in both extenders, particularly 10% PRP, improved the quality and functionality of the sperm in both equilibrated and frozen-thawed semen. Additionally, the antioxidant enzyme activities in both extenders were higher in the PRP-supplemented groups compared to the control group in thawed semen (P < 0.05). All post-thaw sperm quality, antioxidant enzyme activities, and functionality aside from DNA integrity were higher (P < 0.05) in the PRP-supplemented OptiXcell® than in the PRP-supplemented Tris egg yolk-based extender. The fertility of cryopreserved semen in the extenders supplemented with 10% and 15% PRP increased (P < 0.05) significantly more than that of the control extenders, with 10% PRP being the optimum concentration in OptiXcell® (80%) compared to that of Tris egg yolk-based extender (66.67%) and control of two extenders (53.33% and 46.67%, respectively).
CONCLUSIONS: Even though autologous PRP-supplemented extenders have a protective impact on equilibrated and cryopreserved semen, 10% PRP-supplemented OptiXcell® extenders are more effective at preserving post-thaw semen quality, functionality, and antioxidant capacity, which increases the in vivo fertility of buffalo bulls.
RESULTS: All PRP concentrations in both extenders, particularly 10% PRP, improved the quality and functionality of the sperm in both equilibrated and frozen-thawed semen. Additionally, the antioxidant enzyme activities in both extenders were higher in the PRP-supplemented groups compared to the control group in thawed semen (P < 0.05). All post-thaw sperm quality, antioxidant enzyme activities, and functionality aside from DNA integrity were higher (P < 0.05) in the PRP-supplemented OptiXcell® than in the PRP-supplemented Tris egg yolk-based extender. The fertility of cryopreserved semen in the extenders supplemented with 10% and 15% PRP increased (P < 0.05) significantly more than that of the control extenders, with 10% PRP being the optimum concentration in OptiXcell® (80%) compared to that of Tris egg yolk-based extender (66.67%) and control of two extenders (53.33% and 46.67%, respectively).
CONCLUSIONS: Even though autologous PRP-supplemented extenders have a protective impact on equilibrated and cryopreserved semen, 10% PRP-supplemented OptiXcell® extenders are more effective at preserving post-thaw semen quality, functionality, and antioxidant capacity, which increases the in vivo fertility of buffalo bulls.
摘要：
背景：布法罗精子具有独特的膜结构，使它们更容易冷冻保存，导致解冻后精子质量较低。这降低了水牛人工授精的成功率。了解和解决这些特定的脆弱性对于改善水牛种群的生殖技术至关重要。在这项研究中检查了冷冻保存的水牛精液的特性，这些特性涉及将自体富血小板血浆（PRP）添加到OptiXcell®或Tris基于蛋黄的补充剂中的影响。十头水牛被用来收集精液。每只公牛的射精被分成两个主要相等的量，然后用OptiXcell®或Tris蛋黄补充剂稀释，补充各种PRP浓度(5%，10%,和15%)，和对照(0%)，在根据既定方案进行冷冻保存之前。平衡和解冻后，对精子的质量和功能进行了评估，随着抗氧化酶活性（GSH和TAC），丙二醛(MDA)含量,和体内解冻精液的受精率。
结果：两种增量剂中的所有PRP浓度，特别是10%的PRP，提高了平衡精液和冻融精液中精子的质量和功能。此外,与解冻精液中的对照组相比，PRP补充组的两种补充剂中的抗氧化酶活性均较高（P<0.05）。所有解冻后的精子质量，抗氧化酶活性，补充PRP的OptiXcell®比补充PRP的基于Tris蛋黄的延伸剂中除了DNA完整性之外的功能性更高(P<0.05)。补充10％和15％PRP的补充剂中冷冻保存的精液的生育力显着增加（P<0.05）比对照补充剂的生育力增加（P<0.05）。与基于Tris蛋黄的补充剂（66.67%）和两种补充剂的对照（53.33%和46.67%）相比，OptiXcell®中10%PRP是最佳浓度（80%），分别)。
结论：尽管补充了自体PRP的补充剂对平衡和冷冻保存的精液有保护作用，10％PRP补充的OptiXcell®补充剂在保存解冻后精液质量方面更有效，功能，和抗氧化能力，这增加了水牛公牛的体内生育能力。
结果：两种增量剂中的所有PRP浓度，特别是10%的PRP，提高了平衡精液和冻融精液中精子的质量和功能。此外,与解冻精液中的对照组相比，PRP补充组的两种补充剂中的抗氧化酶活性均较高（P<0.05）。所有解冻后的精子质量，抗氧化酶活性，补充PRP的OptiXcell®比补充PRP的基于Tris蛋黄的延伸剂中除了DNA完整性之外的功能性更高(P<0.05)。补充10％和15％PRP的补充剂中冷冻保存的精液的生育力显着增加（P<0.05）比对照补充剂的生育力增加（P<0.05）。与基于Tris蛋黄的补充剂（66.67%）和两种补充剂的对照（53.33%和46.67%）相比，OptiXcell®中10%PRP是最佳浓度（80%），分别)。
结论：尽管补充了自体PRP的补充剂对平衡和冷冻保存的精液有保护作用，10％PRP补充的OptiXcell®补充剂在保存解冻后精液质量方面更有效，功能，和抗氧化能力，这增加了水牛公牛的体内生育能力。
