肿瘤教育的血小板(TEP)已被广泛报道具有有希望的应用潜力;尽管如此,在基于血小板的液体活检的TEP研究中,从外周血中分离血小板是一个重要但被忽视的步骤.在这篇文章中,我们讨论了血小板分离的一些常见影响因素。探讨血小板分离的相关因素,我们对健康的中国汉族成年人(18~79岁)进行了一项前瞻性多中心研究.从四家医院前瞻性招募的226名健康志愿者中,共有208名个体被纳入最终统计分析。主要研究指标是血小板恢复率(PRR)。在四家医院也观察到了类似的模式,室温(23°C±2°C)下的PRR略高于低温(4°C±2°C)下的PRR。此外,随着贮藏时间的增加,PRR逐渐降低。储存2小时内的样品的PRR显著高于超过2小时的样品(p<.05)。此外,PRR也受到不同中心使用的设备的影响。这项研究证实了影响血小板分离的几个因素。在我们的研究中,我们指出,血小板分离应在外周血抽取后两小时内进行,并保持在室温下直至分离,在提取过程中应该固定离心机模型,这将进一步改善基于血小板的液体活检在癌症中的研究进展。
背景是什么?全球范围内,癌症是导致过早死亡的主要原因之一。早期筛查对癌症诊断和治疗非常重要,甚至可以显著降低全球癌症死亡率。癌症是导致过早死亡的主要原因之一。早期筛查对于癌症诊断和治疗很重要,甚至可以显着降低癌症死亡率。对于液体活检,隔离是重要的一步。早期的研究已经探索了外泌体的影响因素,循环肿瘤细胞(CTC),和液体活检中的其他成分提取。尽管血小板也是液体活检的极好来源,很少有研究探讨影响血小板分离的因素。考虑到血小板分离在肿瘤血小板液体活检中的重要性,我们的目标是尽可能优化血小板分离条件,以获得较高的血小板回收率。什么是新的?在这项研究中,我们对来自四个中心的健康成年人进行了前瞻性多中心研究,联合全血和富血小板血浆,探讨血小板分离过程中影响血小板恢复率(PRR)的因素。在我们的研究中,我们指出,血小板分离应在室温下两小时内进行,在提取过程中应该固定离心机模型,这将进一步改善基于血小板的液体活检在癌症中的研究进展。有什么影响?在未来的血小板相关研究中,我们应该固定样品储存温度,血小板提取过程中的储存时间和离心模型,从而尽可能减少影响血小板提取的变量,保证血小板提取的稳定回收率。
Tumor-educated platelets (TEPs) have been widely reported to have promising application potential; nonetheless, platelet isolation from peripheral blood is an important but neglected step in TEPs research for platelet-based liquid biopsy. In this article, we discussed some common influence factors for platelet isolation. To investigate the factors involved in platelet isolation, a prospective multicenter
study was conducted on healthy Han Chinese adults (18 to 79 years of age). A total of 208 individuals were included in the final statistical analysis out of the 226 healthy volunteers who were prospectively enrolled from four hospitals. The primary
study metric was the platelet recovery rate (PRR). The similar pattern was observed in the four hospitals, The PRR at room temperature (23°C±2°C) was slightly higher than the PRR at cold temperature (4°C±2°C). Moreover, the PRR gradually decreased as the storage time increased. The PRR for samples within 2 hours of storage is significantly higher than for samples beyond 2 hours (p < .05). Additionally, PRR was also affected by the equipment used in different centers. This
study confirmed several factors that influence platelet isolation. In our
study, we indicated that platelet isolation should be performed within two hours of peripheral blood draw and held at room temperature until isolation, and that centrifuge models should be fixed during the extraction process, which will further improve the research progress of platelet-based liquid biopsy in cancer.
What is the context? Globally, cancer is one of the leading cause of premature death. Early screening is important for cancer diagnosis and treatment and can even significantly lower cancer mortalityGlobally, cancer is one of the leading cause of premature death. Early screening is important for cancer diagnosis and treatment and can even significantly lower cancer mortalityFor the liquid biopsy, isolation is an important step. Early studies have explored the influencing factors of exosome, circulating tumor cells (CTCs), and other components extraction in liquid biopsy.Despite platelet also being an excellent source of liquid biopsy, few studies have explored the factors that influence platelet isolation.Considering the importance of platelet isolation in tumor-based platelet liquid biopsy, our aim is to optimize platelet isolation conditions as much as possible to obtain a high platelet recovery rate.What is new? In this
study, we conducted a prospective multicenter
study ofhealthy adults from four centers, combining whole blood with platelet-richplasma to investigate factors influencing platelet recovery rate (PRR) during platelet isolation.In our study, we indicated that platelet isolation should be performed within two hours at room temperature, and that centrifuge models should be fixed during the extraction process, which will further improve the research progress of platelet-based liquid biopsy in cancer.What is the impact? In future platelet-related studies, we should fix the sample storage temperature, storage time and centrifuge model in the process of platelet extraction, so as to reduce the variables affecting platelet extraction as much as possible and ensure the stable recovery rate of platelet extraction.