PDF(Pubmed)
Abstract：
There are many potential therapeutic applications for autologous adipose-derived stromal cells. These cells are found in a heterogeneous population isolated from adipose tissue called the stromal vascular fraction (SVF). Closed automated systems are available to release cells from the adherent stroma. Here, we test one system to evaluate the heterogeneous output for yield, purity, cellular characterization, and stemness criteria. The SVF was isolated from three donors using the Automated Cell Station (ACS) from BSL Co., Ltd., Busan, Republic of Korea. The SVF cellular output was characterized for cell yield and viability, immunophenotyping analysis, pluripotent differentiation potential, adhesion to plastic, and colony-forming units. Additionally, the SVF was tested for endotoxin and collagenase residuals. The SVF yield from the ACS system was an average volume of 7.9 ± 0.5 mL containing an average of 19 × 106 nucleated cells with 85 ± 12% viability. Flow cytometry identified a variety of cells, including ASCs (23%), macrophages (24%), endothelial cells (5%), pericytes (4%), and transitional cells (0.5%). The final concentrated product contained cells capable of differentiating into adipogenic, chondrogenic, and osteogenic phenotypes. Furthermore, tests for SVF sterility and purity showed no evidence of endotoxin or collagenase residuals. The ACS system can efficiently process cells from adipose tissue within the timeframe of a single surgical procedure. The cellular characterization indicated that this system can yield a sterile and concentrated SVF output, providing a valuable source of ASCs within the heterogeneous cell population.
摘要：
自体脂肪来源的基质细胞有许多潜在的治疗应用。这些细胞存在于从脂肪组织分离的称为基质血管部分(SVF)的异质群体中。封闭的自动化系统可用于从贴壁基质释放细胞。这里,我们测试一个系统来评估异质输出的产量，纯度,细胞表征，和干性标准。使用BSL公司的自动细胞站（ACS）从三个供体中分离出SVF。Ltd.,釜山,大韩民国。SVF细胞输出被表征为细胞产量和活力,免疫表型分析，多能分化潜能，对塑料的附着力，和菌落形成单位。此外,对SVF进行内毒素和胶原酶残留检测.来自ACS系统的SVF产量是7.9±0.5mL的平均体积,含有平均19×106个有核细胞,具有85±12%的活力。流式细胞术鉴定了多种细胞，包括ASC（23%），巨噬细胞（24%），内皮细胞（5%），周细胞（4%），和过渡细胞（0.5％）。最终的浓缩产物含有能够分化为脂肪的细胞，软骨形成,和成骨表型。此外，对SVF无菌性和纯度的测试显示没有内毒素或胶原酶残留的证据。ACS系统可以在单个外科手术的时间范围内有效地处理来自脂肪组织的细胞。细胞表征表明，该系统可以产生无菌和浓缩的SVF输出，在异质细胞群内提供有价值的ASC来源。
