Broomcorn小米(BM),最早起源于中国北方的驯化粮食作物之一,可以忍受极端条件,比如干旱和高温,在盐碱中普遍存在,干旱,和贫瘠的风景。然而,其对碱胁迫的适应机制尚待全面了解。在这项研究中,80和40mM标准碱应力浓度用于,分别,评价296种BM基因型在萌发和幼苗阶段的耐碱性。主成分分析(PCA),皮尔逊相关分析,和F值综合分析了发芽参数(发芽势,发芽指数,发芽率,活力指数,根长/重量,发芽长度/重量,和碱损坏率)。根据它们各自的F值,BM基因型分为高耐碱到碱敏感五类。为研究幼苗对碱胁迫的响应,我们调查了表型参数(植物高度,绿叶面积,生物量,和根结构)来自上述五类的111个基因型。结合发芽和幼苗期的耐碱参数,这111个基因型被进一步细分为具有不同耐碱性的三组.进一步研究了不同耐碱基因型的生理反应变化的抗氧化酶活性,可溶性物质,丙二醛(MDA)含量,电解质泄漏率,和叶子结构。与碱敏感基因型相比,耐碱基因型具有较高的抗氧化酶活性和可溶性渗透压物质含量,低MDA含量和电解质泄漏率,和更完整的气孔结构。一起来看,本研究为评价耐碱性提供了一种全面可靠的方法,将有助于BM对盐碱土壤的改良和修复。
Broomcorn millet (BM), one of the earliest domesticated cereal crops originating in northern China, can tolerate extreme conditions, such as drought and high temperatures, which are prevalent in saline-alkali, arid, and barren landscapes. However, its adaptive mechanism to alkali stress is yet to be comprehensively understood. In this study, 80 and 40 mM standard alkali stress concentrations were used to, respectively, evaluate the alkali tolerance at the germination and seedling stages of 296 BM genotypes. Principal component analysis (PCA), Pearson\'s correlation analysis, and F-value comprehensive analysis were performed on the germination parameters (germination potential, germination index, germination rate, vigor index, root length/weight, sprout length/weight, and alkali damage rate). Based on their respective F-values, the BM genotypes were divided into five categories ranging from highly alkali resistant to alkali sensitive. To study the response of seedlings to alkaline stress, we investigated the phenotypic parameters (plant height, green leaf area, biomass, and root structure) of 111 genotypes from the above five categories. Combining the parameters of alkali tolerance at the germination and seedling stages, these 111 genotypes were further subdivided into three groups with different alkali tolerances. Variations in physiological responses of the different alkali-tolerant genotypes were further investigated for antioxidant enzyme activity, soluble substances, malondialdehyde (MDA) content, electrolyte leakage rate, and leaf structure. Compared with alkali-sensitive genotypes, alkali-tolerant genotypes had high antioxidant enzyme activity and soluble osmolyte content, low MDA content and electrolyte leakage rate, and a more complete stomata structure. Taken together, this study provides a comprehensive and reliable method for evaluating alkali tolerance and will contribute to the improvement and restoration of saline-alkaline soils by BM.