羊痘是由羊痘病毒羊痘病毒感染引起的小反刍动物的跨界疾病。羊痘在非洲发现,中东和亚洲的特点是发烧,多灶性皮肤隆起性病变和死亡。用减毒活羊痘病毒(CPPV)毒株接种疫苗是控制羊痘暴发的有效且广泛使用的策略;但是,很少有疫苗接种后现场监测研究的报告.这项研究使用了市售的酶联免疫吸附测定(ELISA)来检查蒙古用减毒活CPPV菌株接种的绵羊的体液反应的定量和时间特征。使用ELISA商业试剂盒测试了400个样品,和45个样品的子集也用病毒中和测试(VNT)。在VNT和ELISA测试之间存在实质的一致性。在疫苗接种后40至262天之间检测到针对CPPV的抗体。血清学状态(阳性/阴性)与性别或年龄之间没有显着差异;但是,疫苗接种后的时间长度与血清学状态呈负相关.在疫苗接种后90至180天之间的动物比疫苗接种后大于180天的动物更可能是阳性的。我们的结果表明,商业CPPVELISA试剂盒是在资源受限的环境中进行CPPV疫苗接种后监测的可靠可靠方法,并提供了计划羊痘疫苗接种后监测计划时要考虑的时间参数。
Sheeppox is a transboundary disease of small ruminants caused by infection with the
capripoxvirus sheeppox virus. Sheeppox is found in Africa, the Middle East and Asia and is characterized by fever, multifocal cutaneous raised lesions and death. Vaccination with live attenuated
capripoxvirus (CPPV) strains is an effective and widely used strategy to contol sheeppox outbreaks; however, there are few reports of post-vaccination field surveillance studies. This
study used a commercially available enzyme-linked immunosorbent assay (ELISA) to examine quantitative and temporal features of the humoral response of sheep vaccinated with a live-attenuated CPPV strain in Mongolia. Four hundred samples were tested using the ELISA commercial kit, and a subset of 45 samples were also tested with a virus neutralization test (VNT). There was substantial agreement between the VNT and ELISA tests. Antibodies to CPPV were detected between 40 and 262 days post-vaccination. There was no significant difference between serological status (positive/negative) and sex or age; however, an inverse correlation was found between the length of time since vaccination and serological status. Animals between 90 and 180 days post-vaccination were more likely to be positive than animals greater than 180 days post-vaccination. Our results show that a commercial CPPV ELISA kit is a robust and reliable assay for post-CPPV vaccination surveillance in resource-restricted settings and provide temporal parameters to be considered when planning sheeppox post-vaccination monitoring programmes.