■瞬时受体电位香草素1(TRPV1)是一种热激活的非选择性阳离子通道,在精子发生中起重要作用,获能,顶体反应和精子/卵母细胞融合。考虑到精索静脉曲张条件下睾丸温度高和氧化应激,我们旨在评估不育男性精子中TRPV1的表达。
■在本病例对照研究中,招募了25名患有精索静脉曲张(II级和III级)的男性以及25名可育者。精子参数,鱼精蛋白缺乏症(色霉素A3),DNA损伤(TUNEL),脂质过氧化(BODIPY),TRPV1基因表达(实时聚合酶链反应),TRPV1蛋白(流式细胞术和免疫细胞化学技术),评估可育和精索静脉曲张组之间的顶体反应。
■我们观察到精子参数显着下降,而且,DNA损伤增加,脂质过氧化,精索静脉曲张组鱼精蛋白缺乏。虽然,TRPV1的mRNA表达在精索静脉曲张和可育组之间相似,与可育组相比,精索静脉曲张组的蛋白质表达显着降低。此外,TRPV1定位从赤道区域改变到头部的顶体区域,尤其是在顶体区域,在诱导顶体反应后,可育组比精索静脉曲张组更显著。
■除了精子参数的质量,精索静脉曲张组的染色质完整性明显降低,在精索静脉曲张条件下,TRPV1蛋白的表达也较低,这可能与获能减少有关,顶体反应和精子/卵母细胞融合,从而导致不育。
UNASSIGNED: Transient receptor potential vanilloid 1 (TRPV1) is a heat-activated nonselective cation channel that plays important role in the spermatogenesis,
capacitation, acrosome reaction and sperm/oocyte fusion. Considering the high testicular temperature and oxidative stress in varicocele condition, we aimed to assess expression of TRPV1 in sperm of infertile men.
UNASSIGNED: In this case-control
study, twenty-five men with varicocele (grade II and III) as well as twentyfive fertile were recruited. Sperm parameters, protamine deficiency (Chromomycin A3), DNA damage (TUNEL), lipid peroxidation (BODIPY), TRPV1 gene expression (real time polymerase chain reaction), TRPV1 protein (flowcytometry and immunocytochemical techniques), and acrosome reaction were assessed between fertile and varicocele groups.
UNASSIGNED: We observed a significant decrease in the sperm parameters, and also, an increased DNA damage, lipid peroxidation, and protamine deficiency in varicocele group. Although, the mRNA expression of TRPV1 was similar between varicocele and fertile groups, its expression at the protein level was significantly decreased in the varicocele group in comparison with fertile group. Additionally, the TRPV1 localization was changed from the equatorial region to the acrosomal region of the head, especially in the acrosomal region, which was more significant in the fertile group than the varicocele group after inducing acrosome reaction.
UNASSIGNED: In addition to the quality of sperm parameters, and chromatin integrity that were lower significantly in varicocele group, the expression of TRPV1 protein was also lower in varicocele condition that could be associated with reduced
capacitation, acrosome reaction and sperm/oocyte fusion and thereby infertility.