开发了具有优异荧光性能的近红外荧光团,一个大的斯托克斯位移,良好的生物相容性已成为近年来荧光成像领域的研究热点。基于量子化学计算和合理的分子设计策略,开发了一种新的近红外荧光团,其特征在于合成简单,易于结构修改,和大的斯托克斯位移(105nm)。此外,使用简单的结构修饰合成了两种新的“可激活”荧光探针QN-Cys和QN-DNP。探针QN-Cys可以高灵敏度(LOD=128nM)和高选择性识别Cys,其荧光强度在5~35μM范围内与Cys浓度呈良好的线性关系。此外,QN-Cys探针能有效区分Cys与Hcy和GSH,并成功应用于人血清中Cys的检测和成像,细胞,还有斑马鱼.探针QN-DNP对苯硫酚表现出良好的特异性和敏感性(LOD=78nM)荧光响应,其荧光强度在5~30μM范围内与苯硫酚浓度呈良好的线性关系。此外,成功应用于实际水样中苯硫酚的检测,回收率(97-102%),在活细胞中成像苯硫酚,斑马鱼和老鼠。值得注意的是,QN-DNP探针可用于观察苯硫酚在小鼠体内的分布。
The development of a near-infrared fluorophore with excellent fluorescence performance, a large Stokes shift, and good biocompatibility has become a focus in the field of fluorescence imaging in recent years. Based on quantum chemistry calculations and reasonable molecular design strategies, a new NIR fluorophore was developed and characterized by simple synthesis, easy structural modification, and a large Stokes shift (105 nm). Furthermore, two new \"activatable\" fluorescent probes QN-Cys and QN-DNP were synthesized using a simple structural modification. The probe QN-Cys can recognize Cys with high sensitivity (LOD = 128 nM) and high selectivity, and its fluorescence intensity has a good linear relationship with the Cys concentration in the range of 5-35 μM. Furthermore, probe QN-Cys can effectively distinguish Cys from Hcy and GSH, and was successfully applied to the detection and imaging of Cys in human serum, cells, and zebrafish. The probe QN-DNP showed a good specific and sensitive (LOD = 78 nM) fluorescence response to thiophenol, and its fluorescence intensity has a good linear relationship with the thiophenol concentration in the range of 5-30 μM. Furthermore, it was successfully applied to detect thiophenol in real water samples with good recoveries (97-102%), and image thiophenol in living cells, zebrafish and mice. Notebly, the QN-DNP probe could be applied to visualize the distribution of thiophenol in the mice.