目的:B7分子(B7s)是针对肿瘤细胞的有效免疫监视的关键协同信号。虽然以前的研究已经探讨了B7家族和癌症之间的关联,大多数仅限于特定基因或癌症亚型。
方法:我们的研究利用多组学数据来研究B7s表达之间的潜在相关性(B7sexpr。)和预后,临床病理特征,体细胞突变(SM),拷贝数变化(CNVs),免疫特性,肿瘤微环境(TME),微卫星不稳定,肿瘤突变负荷,免疫检查点基因(ICG),和TCGA肿瘤中的药物反应性。此外,B7sexpr之间的连接。和免疫治疗(IT)性能在各种验证的数据集中评估。在此之后,基于B7sexpr进行免疫浸润分析(IIA)。,CNVs,或膀胱癌(BLCA)中的SM,通过实时PCR(RT-PCR)和B7-H3的蛋白质确认进行补充。
结果:在大多数癌症类型中,B7sexpr.与预后有关,临床病理特征,突变,CNVs,ICG,TMB,TME.对抗癌药物敏感性的检查揭示了B7分子与不同药物敏感性之间的相关性。特定的B7sexpr。模式与IT的临床有效性有关。使用GSEA,鉴定了几种富集的免疫相关功能和途径.特别是在BLCA,IIA揭示了B7CNVs之间的显著联系,突变状态,和各种免疫细胞浸润。RT-PCR证实BLCA肿瘤组织中B7-H3基因水平升高。
结论:本研究证实了B7sexpr的意义。和基因组变化在预测不同癌症类型的结果和治疗。此外,它们表明B7在BLCA中的关键功能及其作为IT生物标志物的潜力。
OBJECTIVE: B7 molecules (B7s) are crucial synergistic signals for effective immune surveillance against tumor cells. While previous studies have explored the association between the B7 family and cancer, most have been limited to specific genes or cancer subtypes.
METHODS: Our study utilized multi-omics data to investigate potential correlations between B7s expression (B7s exp.) and prognosis, clinicopathological features, somatic mutations (SMs), copy number variations (CNVs), immune characteristics, tumor microenvironment (TME), microsatellite instability, tumor mutation burden, immune checkpoint gene (ICG), and drug responsiveness in TCGA tumors. Furthermore, the connection between B7s exp. and immunotherapy (IT) performance assessed in various validated datasets. Following this, immune infiltration analysis (IIA) was conducted based on B7s exp., CNVs, or SMs in bladder cancer (BLCA), complemented by real-time PCR (RT-PCR) and protein confirmation of B7-H3.
RESULTS: Across most cancer types, B7s exp. was related to prognosis, clinicopathological characteristics, mutations, CNVs, ICG, TMB, TME. The examination of sensitivity to anticancer drugs unveiled correlations between B7 molecules and different drug sensitivities. Specific B7s exp. patterns were linked to the clinical effectiveness of IT. Using GSEA, several enriched immune-related functions and pathways were identified. Particularly in BLCA, IIA revealed significant connections between B7 CNVs, mutation status, and various immune cell infiltrates. RT-PCR confirmed elevated B7-H3 gene levels in BLCA tumor tissues.
CONCLUSIONS: This study confirmed the significance of B7s exp. and genomic changes in predicting outcomes and treatment across different cancer types. Moreover, they indicate a critical function of B7s in BLCA and their potential as IT biomarkers.