凝血酶是二次止血生物化学中的关键酶,对维持止血的稳态至关重要。与常规凝血测试(PT或aPTT)或促凝血或抗凝血因子测定(例如纤维蛋白原,因子VIII,抗凝血酶或蛋白C),凝血酶生成测定(TGA),也称为凝血酶生成测试(TGT)是一种所谓的“全局测定”,通过连续和同时测量凝血酶的形成和抑制来提供止血平衡的图片。最早描述于1950年代初,作为手动测定,已经做出努力,以标准化和自动化检测,为研究人员提供,临床实验室和制药行业的多功能工具,涵盖了广泛的临床和非临床应用。这篇综述描述了为正确运行TGA而提供的技术选项,包括对分析前和分析项目的审查,性能,解释,以及在生理学研究和药学中的应用。
Thrombin is the pivotal enzyme in the biochemistry of secondary hemostasis crucial to maintaining homeostasis of hemostasis. In contrast to routine coagulation tests (PT or aPTT) or procoagulant or anticoagulant factor assays (e.g. fibrinogen, factor VIII, antithrombin or protein C), the thrombin generation assay (TGA), also named thrombin generation test (TGT) is a so-called \"global assay\" that provides a picture of the hemostasis balance though a continuous and simultaneous measurement of thrombin formation and inhibition. First described in the early 1950s, as a manual assay, efforts have been made in order to standardize and automate the assay to offer researchers, clinical laboratories and the pharmaceutical industry a versatile tool covering a wide range of clinical and non-clinical applications. This
review describes technical options offered to properly run TGA, including a
review of preanalytical and analytical items, performance, interpretation, and applications in physiology research and pharmacy.