背景:麻风树属(大齿科)的物种在传统医学中不加区别地用于治疗涉及有毒动物的事故。麻疯树.,俗称“pinhão-de-seda”,\“在巴西东北部的半干旱地区发现。它被广泛用作蠕虫,去净化,泻药,和抗蛇毒血清.
目的:获得麻疯树(Jmla)乳胶的植物化学特征,并评估其急性经口毒性和对蝎子TityusStigmurus(TstiV)毒液的抑制作用。
方法:通过茎中的原位切口获得J.mutabilis(Jmla)的乳胶,并使用HPLC-ESI-QToF-MS进行表征。在小鼠中研究急性口服毒性。通过电泳获得了T.stigmurus毒液的蛋白质谱。使用SDS-PAGE评估乳胶与毒液成分(TstiV)相互作用的能力,UV-VIS扫描光谱,以及纤溶和透明质酸酶活性的中和。此外,在体内评估乳胶抑制由毒液引起的局部致水肿和伤害性作用的能力。
结果:乳胶的植物化学特征显示存在75种化合物,包括环状肽,糖苷,酚类化合物,生物碱,香豆素,和萜类化合物,在其他人中。在2000mg/kg(p.o.)的剂量下没有观察到急性毒性的迹象。胶乳与TstiV的蛋白质谱相互作用,100%抑制毒液的纤溶和透明质酸酶活性。此外,乳胶能够减轻局部毒害效应,与阴性对照组相比,伤害感受减少高达56.5%,水肿减少高达50%。
结论:麻疯树的乳胶表现出多样化的植物化学成分,含有许多种类的代谢物。它在小鼠中不存在急性毒性作用,并且具有在体外抑制Tityusstigmurus毒的酶促作用的能力。此外,它减少体内的伤害性感受和水肿。这些发现证实了有关该植物抗蛇毒血清活性的流行报道,并表明该乳胶具有治疗蝎子病的潜力。
BACKGROUND: Species of the Jatropha genus (Euphorbiaceae) are used indiscriminately in traditional medicine to treat accidents involving venomous animals. Jatropha mutabilis Baill., popularly known as \"pinhão-de-seda,\" is found in the semi-arid region of Northeastern Brazil. It is widely used as a vermifuge, depurative, laxative, and
antivenom.
OBJECTIVE: Obtaining the phytochemical profile of the latex of Jatropha mutabilis (JmLa) and evaluate its acute oral toxicity and inhibitory effects against the venom of the scorpion Tityus stigmurus (TstiV).
METHODS: The latex of J. mutabilis (JmLa) was obtained through in situ incisions in the stem and characterized using HPLC-ESI-QToF-MS. Acute oral toxicity was investigated in mice. The protein profile of T. stigmurus venom was obtained by electrophoresis. The ability of latex to interact with venom components (TstiV) was assessed using SDS-PAGE, UV-Vis scanning spectrum, and the neutralization of fibrinogenolytic and hyaluronidase activities. Additionally, the latex was evaluated in vivo for its ability to inhibit local edematogenic and nociceptive effects induced by the venom.
RESULTS: The phytochemical profile of the latex revealed the presence of 75 compounds, including cyclic peptides, glycosides, phenolic compounds, alkaloids, coumarins, and terpenoids, among others. No signs of acute toxicity were observed at a dose of 2000 mg/kg (p.o.). The latex interacted with the protein profile of TstiV, inhibiting the venom\'s fibrinogenolytic and hyaluronidase activities by 100%. Additionally, the latex was able to mitigate local envenomation effects, reducing nociception by up to 56.5% and edema by up to 50% compared to the negative control group.
CONCLUSIONS: The latex of Jatropha mutabilis exhibits a diverse phytochemical composition, containing numerous classes of metabolites. It does not present acute toxic effects in mice and has the ability to inhibit the enzymatic effects of Tityus stigmurus venom in vitro. Additionally, it reduces nociception and edema in vivo. These findings corroborate popular reports regarding the
antivenom activity of this plant and indicate that the latex has potential for treating scorpionism.