OBJECTIVE: Determination of age is a pivotal part of forensic dentistry. Since many dental age determination methods are invasive, Cameriere introduced a radiographic method where age evaluation was carried out using an orthopantomogram (OPG). An adaptation of the same formula was later formulated by Rai et al. for the Indian population. The aim of the study was to assess the age and to dictate the efficiency of the Indian version of Cameriere\'s formula in the Chennai subpopulation.
METHODS: This study consisted of radiographs belonging to 50 individuals with an age range of 5-15 years. Radiographs were taken using the OPG and the soft copies obtained were analyzed. The variables such as seven left and right permanent mandibular teeth and the number of teeth with closed apical, and with open apical, ends of roots were examined and measured. The obtained data, after substituting in the formula was statistically analyzed using paired and unpaired t-test and Pearson\'s correlation coefficient test.
RESULTS: On comparing dental age and chronological age, statistically significant results were obtained on both sides of the radiograph. Between genders, both sides of the radiograph showed non-significant results. On correlating the sides, the left side showed greater accuracy in age determination compared to the right side of the OPG.
CONCLUSIONS: Age estimation using Indian-specified formula provides a near good estimate and hence can be a reliable method to measure the chronological age of the participants.
CONCLUSIONS: The Indian version of the Cameriere\'s formula can be used as a rational tool to assess the age of young children and adolescents.

The Christchurch groundwater system is an exceptional water resource with very high drinking water quality supplying all the water requirements of the city. The groundwater system has changed over the years because of rising groundwater abstraction due to increasing population and development. The present (2017) data revealed slightly older 14C ages and increasingly steep west-east age gradients compared to the earlier work from 1976 to 2006, showing continued upflow of deep water into the exploited aquifers which is much older on the east (coastal) side than on the west (inland) side. In addition, the 3H ages for wells on the west side of the system are often much younger than their 14C ages showing that there is input of young shallow water to the wells in addition to the deep water input. Application of a binary model identifies the ages and amounts of the two components, showing that the young component is becoming younger although smaller as a proportion of the flow, and the old component from depth is becoming larger. Newly completed wells near the Waimakariri River have allowed identification of the young component, which is almost entirely composed of very young Waimakariri River seepage at all depths and therefore has very little chemical loading. Instead any chemical input (e.g. chloride, nitrate) to the Christchurch aquifers is being brought in by the old deep component which on the western side of the Christchurch system is derived from rainfall recharge on the developing Ashley-Waimakariri Plains area (plus river seepage). Chemical traces of this deep input from the northwest are at present very subtle, although more appreciable signals are seen in some wells further to the north of Christchurch. In the future, slowly increasing chemical input to the Christchurch aquifers on the west side of the system is to be expected as abstraction increases.

UNASSIGNED: DNA methylation (DNAm) age can be used to evaluate the chronological age of individuals often called \"epigenetic age.\" In this study, buccal scrape samples were used for the determination of epigenetic age.
UNASSIGNED: To examine if epigenetic age could be determined using neuronal pentraxin 2 (NPTX2) gene in buccal cells.
UNASSIGNED: This cohort study was designed to validate the use of buccal cells for epigenetic age estimation. Sanger sequencing was used to determine the genetic sequence of the gene of interest postamplification. Nucleotide base sequence for NPTX2 gene was obtained for each case using this protocol.
UNASSIGNED: The study was conducted on buccal scrapes obtained from 26 subjects of both genders, whose age varied from 1 to 65 years. The samples, collected by wooden spatulas, were placed in cell suspension buffer and stored at 4°C until transported to the laboratory.
UNASSIGNED: Methylation levels of 5\'-C-phosphate-G-3\' located in the gene NPTX2 of 26 subjects were studied and analyzed by bisulfate sequencing. The percentage of methylation in this study falls in the range between 15% and 51%.
UNASSIGNED: In this study, a sufficient amount of gDNA was retrieved from the buccal cells, thus confirming that buccal scrape was a feasible technique to obtain ample DNA. This study also showed that DNAm-polymerase chain reaction method was a feasible method for the evaluation of methylation pattern of NPTX2 gene.

UNASSIGNED: Age estimation in forensics employ various methods of which Raman microspectroscopy provides a noninvasive method by assessing various parts of dentin.
UNASSIGNED: The aim of this study is to ascertain the age of carious tooth using Raman spectra of apical dentin and to correlate the similarity of the spectra in a defined age group.
UNASSIGNED: Teeth of known age from Indian population (n=48) and morphology (incisors, canine, premolars, molars) indicated for extraction due to caries were allocated into four groups, i.e., Group A (21-30 years), Group B (31-40 years), Group C (41-50 years) and Group D (51-60 years).
UNASSIGNED: Teeth were sectioned and the apical 2 mm of dentin was examined by a Raman microspectroscopy machine for spectra from 400 cm-1 to 1500 cm-1, and peak at phosphate group at 963 cm-1 was taken for statistical analysis.
UNASSIGNED: The data were analyzed using IBM SPSS version 20.0.
UNASSIGNED: Pearson\'s correlation to test the strength of linear relation of the spectra of the teeth within an age group showed an \"r\" value approaching 1. One-way ANOVA to test the difference between means of spectrum values obtained between the four age groups was statistically significant at P < 0.05.
UNASSIGNED: Raman spectroscopic analysis of apical dentin of teeth affected by caries can also be used to estimate the age and the Raman spectra obtained differed for different age groups, and the same can be advocated as an alternative method to ascertain age in forensic dentistry.

The aim of this study was to verify Cameriere\'s regression formula to predict chronologic age in Saudi Population and if proven to be inaccurate, develop a new algorithm specific for Saudi children. A total of 788 Orthopantamographs(495 boys and 293 girls) were analyzed. The tooth measurement parameters were: number of teeth with closed apex (N0), open apex width (a), crown length (l), A/L ratio for each tooth (x1to x7) and summation of all open apex width (s). The radiographic measurements were substituted in the formula: Age = 8.971 + 0.375g + 1.631 x5 + 0.674 N0 - 1.034s-0.176s * N0 The mean dental age assessed by Cameriere\'s method was significantly lower than chronologic age in Saudi population and with both genders and total study population (p < 0.05). A stepwise linear regression showed all parameters gender, N0, x1 to x7 except x2significantly associated with age (R2 = 0.6334, F (6,779) = 168.28, p < 0.05). There was no statistically significant difference between predicted and actual chronologic age for 788 children using regression equation for Saudi population (p = 1). Cameriere\'s formula verified in Saudi children was found to underestimate the chronologic age in both genders. A prediction formula based on Cameriere\'s method specific to Saudi children has been developed for estimating chronologic age. The new regression formula developed will be more accurate for age assessment in Saudi children.

The use of DNA methylation (DNAm) for chronological age determination has been widely investigated within the last few years for its application within the field of forensic genetics. The majority of forensic studies are based on blood, saliva, and buccal cell samples, respectively. Although these types of samples represent an extensive amount of traces found at a crime scene or are readily available from individuals, samples from other tissues can be relevant for forensic investigations. Age determination could be important for cases involving unidentifiable bodies and based on remaining soft tissue e.g. brain and muscle, or completely depend on hard tissue such as bone. However, due to the cell type specificity of DNAm, it is not evident whether cell type specific age-dependent CpG positions are also applicable for age determination in other cell types. Within this pilot study, we investigated whether 13 previously selected age-dependent loci based on whole blood analysis including amongst others ELOVL2, TRIM59, F5, and KLF14 also have predictive value in other forensically relevant tissues. Samples of brain, bone, muscle, buccal swabs, and whole blood of 29 deceased individuals (age range 0-87 years) were analyzed for these 13 age-dependent markers using massive parallel sequencing. Seven of these loci did show age-dependency in all five tissues. The change of DNAm during lifetime was different in the set of tissues analyzed, and sometimes other CpG sites within the loci showed a higher age-dependency. This pilot study shows the potential of existing blood DNAm markers for age-determination to analyze other tissues than blood. We identified seven known blood-based DNAm markers for use in muscle, brain, bone, buccal swabs, and blood. Nevertheless, a different reference set for each tissue is needed to adapt for tissue-specific changes of the DNAm over time.

BACKGROUND: Human skeleton develops from separate ossification centres which continue to ossify till the bone is completely formed. Radiological techniques are very reliable and useful method for estimating the age of individual for forensic and criminal reasons by observing these ossification centres. External inspection for age determination is liable to error. This study is thus aimed to assess the variation in age of fusion of ischial tuberosity in Pakistani population.
METHODS: It was a cross sectional study, wherein data was retrospectively collected at Bahawalpur Victoria Hospital, a tertiary referral centre in which consecutively selected 47 females and 121 males between 10-24 years of age, attending the outpatient, referred from National Database and Registration Authority for the confirmation of age were selected.
RESULTS: There were a total of 13 cases in stage I, 98 in stage II, 23 in stage III and 34 in stage IV. In stage II maximum numbers of cases were between the ages of 19-22 years whereas in stage IV the maximum numbers of cases were between 21-24 years of age.
CONCLUSIONS: It is concluded that the earliest appearance of epiphyseal centre in males occurred at 12-13 years and in females at 10-11 years. While earliest complete union was seen at the age of 19-20 years in females and 16-17 years in males. All cases in age group of 23-24 years showed complete union.

Determining the order of events in a criminal investigation can be crucial in many ways. Although the use of fingermarks is one of the most important forensic tools as a modality of individualisation, placing them in time is a long lasting difficulty. In the study presented in this paper we describe the effects of light and the temperature on depositions of standard solutions containing squalene and cholesterol on paper. The results obtained give a clear view on what type of processes take place during a temperature and a light influenced degradation of the two materials in this study. Using well known methods for the determination of reaction constants it was determined that the degradation mechanism of cholesterol is following a radical route, where the decay of squalene is more affected by temperature.