对抑制乙酰乳酸合酶（ALS）的除草剂的抗性比任何其他除草剂组都以更快的速度增加。这些病例中的绝大多数是由于ALS基因中的各种单核苷酸多态性赋予靶位点抗性。为了确认抗性并帮助生产者采用最佳管理策略，已经设计了许多诊断技术。DNA技术的最新进展加上序列信息的知识已经允许开发准确和快速的诊断测试。虽然整个基于植物的诊断技术，如幼苗生物测定或基于酶的体外生物测定提供准确的结果，它们往往是劳动力和/或空间密集型的，只会对测试的特定除草剂做出反应，使交叉电阻模式的分辨率更加困难。基于DNA的ALS抑制剂耐药性的成功诊断已经通过三种主要技术实现，(1)限制性片段长度多态性,(2)聚合酶链反应扩增特异性等位基因和(3)变性高效液相色谱。所有基于DNA的技术都是相对快速的,并且提供引起抗性的突变的清楚鉴定。基于非靶标机制的抗性未通过这些基于DNA的方法鉴定；然而，考虑到基于目标部位的ALS抑制剂耐药的患病率，这是一个小小的不便。
Resistance to herbicides inhibiting acetolactate synthase (ALS) has been increasing at a faster rate than in any other herbicide group. The great majority of these cases are due to various single-nucleotide polymorphisms in the ALS gene endowing target site resistance. Many diagnostic techniques have been devised in order to confirm resistance and help producers to adopt the best management strategies. Recent advances in DNA technologies coupled with the knowledge of sequence information have allowed the development of accurate and rapid diagnostic tests. While whole plant-based diagnostic techniques such as seedling bioassays or enzyme-based in vitro bioassays provide accurate results, they tend to be labour- and/or space-intensive and will only respond to the particular herbicides tested, making resolution of cross-resistance patterns more difficult. Successful DNA-based diagnosis of ALS inhibitor resistance has been achieved with three main techniques, (1) restriction fragment length polymorphism, (2) polymerase chain reaction amplification of specific alleles and (3) denaturing high-performance liquid chromatography. All DNA-based techniques are relatively rapid and provide clear identification of the mutations causing resistance. Resistance based on non-target mechanisms is not identified by these DNA-based methods; however, given the prevalence of target site-based ALS inhibitor resistance, this is a minor inconvenience.
