Mesh : Anilino Naphthalenesulfonates Fluorescent Dyes Fluorometry Microscopy, Electron Mycobacteriophages / physiology ultrastructure Mycobacterium fortuitum / virology Mycobacterium smegmatis / virology Spectrum Analysis / methods

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Abstract:
According to electron-microscopic data, various cells in the M. smegmatis ATCC607 population interact differently with phage MTPH11. Fluorometric studies of phage-host interactions were performed using a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfonate (ANS). Changes in the electric characteristics of mycobacterial cells infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spectroscopy for rapid phage typing of mycobacteria is discussed.
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