Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella.

随机扩增多态性 DNA 分析和肠细菌重复基因间共有 PCR 在沙门氏菌流行病学研究中的比较。

• 发表时间: Jun 1996
• 来源期刊: FEMS Immunol Med Microbiol
• DOI: 10.1111/j.1574-695X.1996.tb00279.x
• PMID: 8809548
• 文章类型: Comparative Study
• 中科院分区: Q3;Q2
• 方向: 医学
• 作者列表: Millemann Y,Lesage-Descauses MC,Lafont JP,Chaslus-Dancla E

Mesh: Animals; Bacterial Typing Techniques; Evaluation Studies as Topic; France / epidemiology; Polymerase Chain Reaction / methods; Poultry Diseases / epidemiology microbiology; Random Amplified Polymorphic DNA Technique; Salmonella / classification genetics; Salmonella Infections, Animal / epidemiology microbiology; Salmonella enteritidis / classification genetics; Salmonella typhimurium / classification genetics

Abstract

Discrimination of 70 Salmonella strains previously studied by ribotyping was realized by RAPD and ERIC-PCR analysis. RAPD results on the 56 S. typhimurium isolates did not closely match those of ribotyping. With ERIC-PCR, two fingerprints only were obtained. For the 14 S. enteritidis strains, a helpful discrimination was obtained with RAPD analysis, while ERIC-PCR resulted in a single fingerprint.

摘要

通过RAPD和ERIC-PCR分析实现了先前通过病毒分型研究的70株沙门氏菌菌株的鉴别。56种鼠伤寒沙门氏菌分离株的RAPD结果与病毒分型的结果不匹配。用ERIC-PCR,只获得了两个指纹。对于14种肠炎沙门氏菌菌株，通过RAPD分析获得了有益的区分，而ERIC-PCR导致单个指纹。