Increased buffer pH enhances sensitivity and specificity of human papillomavirus detection using consensus primer based PCR.

• 影响指数: 2.623
• 发表时间: Mar 1995
• 来源期刊: J Virol Methods
• DOI: 10.1016/0166-0934(94)00148-a
• PMID: 7769023
• 文章类型: Journal Article
• 中科院分区: Q4
• 方向: 医学
• 作者列表: Payne D,Hoskins S,Schouten H,van Vleuten H,Tyring S

Mesh: Base Sequence; Buffers; Consensus Sequence; DNA Primers; Humans; Hydrogen-Ion Concentration; Magnesium Chloride; Papillomaviridae / classification genetics isolation & purification; Papillomavirus Infections / diagnosis; Phylogeny; Polymerase Chain Reaction / methods; Potassium Chloride / isolation & purification; Sensitivity and Specificity; Tumor Virus Infections / diagnosis

Abstract

Polymerase chain reaction (PCR) buffers were optimized for the specific detection of human papillomavirus (HPV) sequences. The effect of pH, potassium chloride concentration and magnesium chloride concentration of three different consensus primers were examined. Several phylogenetically distinct HPVs (HPV1, HPV2, HPV6, HPV8, HPV16, HPV18, and HPV20) were used to determine the optimal buffer components. Genital types were less sensitive to changes in pH than cutaneous types. Higher buffer pH, with a few exceptions, led to increased sensitivity and specificity of HPV detection.