%0 Journal Article
%T Increased buffer pH enhances sensitivity and specificity of human papillomavirus detection using consensus primer based PCR.
%A Payne D
%A Hoskins S
%A Schouten H
%A van Vleuten H
%A Tyring S
%J J Virol Methods
%V 52
%N 1
%D Mar 1995
%M 7769023
%F 2.623
%R 10.1016/0166-0934(94)00148-a
%X Polymerase chain reaction (PCR) buffers were optimized for the specific detection of human papillomavirus (HPV) sequences. The effect of pH, potassium chloride concentration and magnesium chloride concentration of three different consensus primers were examined. Several phylogenetically distinct HPVs (HPV1, HPV2, HPV6, HPV8, HPV16, HPV18, and HPV20) were used to determine the optimal buffer components. Genital types were less sensitive to changes in pH than cutaneous types. Higher buffer pH, with a few exceptions, led to increased sensitivity and specificity of HPV detection.