Abstract：
BACKGROUND: Fluoroquinolones (FQs) are widely used for their excellent antimicrobial properties, yet their release into aquatic environments pose risks to ecosystems and public health. The accurate monitoring and analysis of FQs present challenges due to their low concentrations and the complex matrices found in actual environmental samples. To address the need for auto-pretreatment and on-line instrumental analysis, developing new microextraction materials and protocols is crucial. Such advancements will provide better analytical assurance for the effective extraction and determination of FQs at trace levels, which is of great significance to environmental protection and human health.
RESULTS: In this work, we presented a Co2+ mediated paper-based molecularly imprinted polymer chip (CMC@Co-MIP), combined with UPLC analysis, to develop an effective analytical method for identifying and quantifying trace amounts of ciprofloxacin (CIP) and enrofloxacin (ENR) in water samples. Notably, the addition of Co2+ in CMC@Co-MIP helped to capture the template molecule CIP through coordination before imprinting, which significantly improved the ordering of the imprinted cavities. CMC@Co-MIP exhibited a maximum adsorption capacity up to 500.20 mg g-1 with an imprinting factor of 4.12, surpassing previous reports by a significant margin. Furthermore, the enrichment mechanism was extensively analyzed by various characterization techniques. The developed method showed excellent repeatability and reproducibility (RSD < 13.0 %) with detection limits ranging from 0.15 to 0.21 μg L-1 and recoveries ranging from 64.9 % to 102.3 % in real spiked water samples.
CONCLUSIONS: We developed a novel microextraction paper-based chip based on Co2+ mediation, which effectively improved the selectivity and convenience of extracting FQs. This breakthrough allowed the chip to have a high enrichment efficiency as well as provide a robust on-line instrumental program. It also confirms that the imprinting scheme based on metal ion coordination is a high-performance strategy.
RESULTS: In this work, we presented a Co2+ mediated paper-based molecularly imprinted polymer chip (CMC@Co-MIP), combined with UPLC analysis, to develop an effective analytical method for identifying and quantifying trace amounts of ciprofloxacin (CIP) and enrofloxacin (ENR) in water samples. Notably, the addition of Co2+ in CMC@Co-MIP helped to capture the template molecule CIP through coordination before imprinting, which significantly improved the ordering of the imprinted cavities. CMC@Co-MIP exhibited a maximum adsorption capacity up to 500.20 mg g-1 with an imprinting factor of 4.12, surpassing previous reports by a significant margin. Furthermore, the enrichment mechanism was extensively analyzed by various characterization techniques. The developed method showed excellent repeatability and reproducibility (RSD < 13.0 %) with detection limits ranging from 0.15 to 0.21 μg L-1 and recoveries ranging from 64.9 % to 102.3 % in real spiked water samples.
CONCLUSIONS: We developed a novel microextraction paper-based chip based on Co2+ mediation, which effectively improved the selectivity and convenience of extracting FQs. This breakthrough allowed the chip to have a high enrichment efficiency as well as provide a robust on-line instrumental program. It also confirms that the imprinting scheme based on metal ion coordination is a high-performance strategy.
摘要：
背景：氟喹诺酮（FQs）因其优异的抗菌性能而被广泛使用，然而，它们释放到水生环境中会对生态系统和公众健康构成风险。FQ的准确监测和分析由于其低浓度和在实际环境样品中发现的复杂基质而面临挑战。为了满足自动预处理和在线仪器分析的需求，开发新的微萃取材料和方案至关重要。这些进步将为有效提取和测定痕量FQ提供更好的分析保证，这对环境保护和人类健康具有重要意义。
结果：在这项工作中，我们提出了一种Co2+介导的纸基分子印迹聚合物芯片(CMC@Co-MIP)，结合UPLC分析，开发一种有效的分析方法，用于鉴定和定量水样中痕量的环丙沙星（CIP）和恩诺沙星（ENR）。值得注意的是,在CMC@Co-MIP中添加Co2+有助于在印迹之前通过配位捕获模板分子P，显着改善了压印腔的有序性。CMC@Co-MIP的最大吸附容量高达500.20mgg-1，印迹因子为4.12，大大超过了以前的报道。此外，通过各种表征技术对富集机理进行了广泛的分析。所开发的方法具有出色的重复性和重现性（RSD<13.0％），在实际加标水样中，检出限为0.15至0.21μgL-1，回收率为64.9％至102.3％。
结论：我们开发了一种基于Co2介导的新型微萃取纸基芯片，有效提高了FQs提取的选择性和便捷性。这一突破使芯片具有高富集效率，并提供了强大的在线仪器程序。这也证实了基于金属离子配位的压印计划是一种高性能的战略。
结果：在这项工作中，我们提出了一种Co2+介导的纸基分子印迹聚合物芯片(CMC@Co-MIP)，结合UPLC分析，开发一种有效的分析方法，用于鉴定和定量水样中痕量的环丙沙星（CIP）和恩诺沙星（ENR）。值得注意的是,在CMC@Co-MIP中添加Co2+有助于在印迹之前通过配位捕获模板分子P，显着改善了压印腔的有序性。CMC@Co-MIP的最大吸附容量高达500.20mgg-1，印迹因子为4.12，大大超过了以前的报道。此外，通过各种表征技术对富集机理进行了广泛的分析。所开发的方法具有出色的重复性和重现性（RSD<13.0％），在实际加标水样中，检出限为0.15至0.21μgL-1，回收率为64.9％至102.3％。
结论：我们开发了一种基于Co2介导的新型微萃取纸基芯片，有效提高了FQs提取的选择性和便捷性。这一突破使芯片具有高富集效率，并提供了强大的在线仪器程序。这也证实了基于金属离子配位的压印计划是一种高性能的战略。
