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Abstract：
Shiga toxin-producing and Enteropathogenic Escherichia coli are foodborne pathogens commonly associated with diarrheal disease in humans. This study investigated the presence of STEC and EPEC in 771 dairy cattle fecal samples which were collected from 5 abattoirs and 9 dairy farms in South Africa. STEC and EPEC were detected, isolated and identified using culture and PCR. Furthermore, 339 STEC and 136 EPEC isolates were characterized by serotype and major virulence genes including stx1, stx2, eaeA and hlyA and the presence of eaeA and bfpA in EPEC. PCR screening of bacterial sweeps which were grown from fecal samples revealed that 42.2% and 23.3% were STEC and EPEC positive, respectively. PCR serotyping of 339 STEC and 136 EPEC isolates revealed 53 different STEC and 19 EPEC serotypes, respectively. The three most frequent STEC serotypes were O82:H8, OgX18:H2, and O157:H7. Only 10% of the isolates were classified as \"Top 7\" STEC serotypes: O26:H2, 0.3%; O26:H11, 3.2%; O103:H8, 0.6%; and O157:H7, 5.9%. The three most frequent EPEC serotypes were O10:H2, OgN9:H28, and O26:H11. The distribution of major virulence genes among the 339 STEC isolates was as follows: stx1, 72.9%; stx2, 85.7%; eaeA, 13.6% and hlyA, 69.9%. All the 136 EPEC isolates were eaeA-positive but bfpA-negative, while 46.5% carried hlyA. This study revealed that dairy cattle are a major reservoir of STEC and EPEC in South Africa. Further comparative studies of cattle and human STEC and EPEC isolates will be needed to determine the role played by dairy cattle STEC and EPEC in the occurrence of foodborne disease in humans.Please kindly check and confirm the country and city name in affiliation [6].This affiliation is correct.Please kindly check and confirm the affiliationsConfirmed. All Affiliations are accurate.
摘要：
产志贺毒素和肠致病性大肠杆菌是食源性病原体，通常与人类腹泻病有关。这项研究调查了从南非的5个屠宰场和9个奶牛场收集的771只奶牛粪便样品中STEC和EPEC的存在。检测到STEC和EPEC,使用培养和PCR分离和鉴定。此外，339个STEC和136个EPEC分离株的特征在于血清型和主要毒力基因，包括stx1，stx2，eaeA和hlyA，以及EPEC中eaeA和bfpA的存在。从粪便样本中培养的细菌扫描的PCR筛选显示，STEC和EPEC阳性的占42.2％和23.3％，分别。339个STEC和136个EPEC分离株的PCR血清分型显示53个不同的STEC和19个EPEC血清型,分别。三种最常见的STEC血清型是O82：H8，OgX18：H2和O157：H7。只有10％的分离株被分类为“前7名”STEC血清型：O26：H2，0.3％；O26：H11，3.2％；O103：H8，0.6％；和O157：H7，5.9％。三种最常见的EPEC血清型是O10：H2，OgN9：H28和O26：H11。339株STEC分离株中主要毒力基因的分布如下:stx1，72.9%；stx2，85.7%；eaeA，13.6%和hlyA，69.9%。所有136个EPEC分离株均为eaeA阳性，但bfpA阴性，而46.5%的人携带hlyA。这项研究表明，奶牛是南非STEC和EPEC的主要水库。需要对牛和人类STEC和EPEC分离株进行进一步的比较研究，以确定奶牛STEC和EPEC在人类食源性疾病发生中的作用。请检查并确认所属国家和城市名称[6]。这种联系是正确的。请检查并确认已确认的关联。所有的关系都是准确的。
