健康的牛,绵羊,山羊可能是胃肠道致病性粪便肠球菌的储库,其中一些可能对抗菌药物具有多重耐药性。这项研究的目的是确定肠球菌在健康绵羊中的流行和多样性,山羊,和牛的尸体,以及分析抗菌药物耐药表型/基因型和毒力基因含量。在2019-2020年期间,从屠宰场的150只反刍动物中收集了car体表面样品。共90种肠球菌,包括五个物种,已获得。肠球菌的总体患病率为60%,其中37.7%被鉴定为肠球菌(E.)hirae,33.3%为卡萨利黄,15.5%为屎肠球菌,12.2%为粪肠球菌,和1.1%的大肠杆菌。efaA的毒力相关基因(12.2%)在肠球菌分离株中普遍存在,其次是gelE(3.3%),asaI(3.3%),和王牌(2.2%)。对喹诺普汀-达福普汀的高抗性(28.8%),四环素(21.1%),氨苄青霉素(20%),和利福平(15.5%)被发现在两个,四,四,和五种肠球菌,分别。确定了肠球菌对11种抗生素组的耐药性,在18.8%的肠球菌中发现了多药耐药(MDR)菌株。通过PCR鉴定了特征抗性基因,发生率为6.6%,2.2%,1.1%,1.1%,1.1%,tetM为1.1%,ermB,ermA,aac(6')Ie-aph(2'')-la,肠球菌分离株中的VanC1和VanC2基因,分别。在肠球菌中检测到导致多药耐药的外排泵基因(34.4%)。结果表明,屠宰场中存在肠球菌,其中许多基因与毒力有关,可能对人体健康有害。
Healthy cattle, sheep, and goats can be reservoirs for gastrointestinal pathogenic fecal enterococci, some of which could be multidrug-resistant to antimicrobials. The objective of this study was to determine the prevalence and diversity of Enterococcus species in healthy sheep, goat, and cattle carcasses, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2019-2020, carcass surface samples were collected from 150 ruminants in a slaughterhouse. A total of 90 enterococci, comprising five species, were obtained. The overall prevalence of enterococci was found to be 60%, out of which 37.7% were identified as Enterococcus (E.) hirae, 33.3% as E. casseliflavus, 15.5% as E. faecium, 12.2% as E. faecalis, and 1.1% as E. gallinarum. Virulence-associated genes of efaA (12.2%) were commonly observed in the Enterococcus isolates, followed by gelE (3.3%), asaI (3.3%), and ace (2.2%). High resistance to quinupristin-dalfopristin (28.8%), tetracycline (21.1%), ampicillin (20%), and rifampin (15.5%) was found in two, four, four, and five of the Enterococcus species group, respectively. The resistance of Enterococcus isolates to 11 antibiotic groups was determined and multidrug resistant (MDR) strains were found in 18.8% of Enterococcus isolates. Characteristic resistance genes were identified by PCR with an incidence of 6.6%, 2.2%, 1.1%, 1.1%, 1.1%, and 1.1% for the tetM, ermB, ermA, aac(6\')Ie-aph(2\")-la, VanC1, and VanC2 genes in Enterococcus isolates, respectively. Efflux pump genes causing multidrug resistance were detected in Enterococcus isolates (34.4%). The results showed that there were enterococci in the slaughterhouse with a number of genes linked to virulence that could be harmful to human health.