Abstract：
Isolation and culture of dorsal root ganglion (DRG) neurons from adult animals is a useful experimental system for evaluating neural plasticity after axonal injury, as well as the neurological dysfunction resulting from aging and various types of disease. In this chapter, we will introduce a detailed method for the culture of mature rat DRG neurons. About 30-40 ganglia are dissected from a rat and mechanically and enzymatically digested. Subsequently, density gradient centrifugation of the digested tissue using 30% Percoll efficiently eliminates myelin debris and non-neuronal cells, to afford neuronal cells with a high yield and purity.
摘要：
从成年动物中分离和培养背根神经节（DRG）神经元是评估轴突损伤后神经可塑性的有用实验系统。以及衰老和各种疾病导致的神经功能障碍。在这一章中,我们将介绍成熟大鼠DRG神经元的详细培养方法。从大鼠中解剖约30-40个神经节并进行机械和酶消化。随后，使用30％Percoll对消化组织进行密度梯度离心，可有效消除髓鞘碎片和非神经元细胞，提供高产量和纯度的神经元细胞。
