Abstract：
Recent advancements in nano- and microfabrication techniques have led to the development of highly biomimetic patterned substrates able to guide neuronal sprouting, routing, elongation, and branching. Such substrates, recapitulating shapes and geometries found in the native brain, may pave the way toward the development of cell instructive paradigms able to guide morphogenesis at the neuron-material interface. In this scenario, high-resolution electron microscopy approaches, owing to their ability of discerning the details of neural morphogenesis at a nanoscale resolution, may play a crucial role in unravelling the fine ultrastructure of neurons interfacing with biomimetic structured substrates.
摘要：
纳米和微加工技术的最新进展导致了能够引导神经元发芽的高度仿生图案化基底的开发，路由,伸长率,和分支。这样的基底，概括在天然大脑中发现的形状和几何形状，可能为能够指导神经元-材料界面形态发生的细胞指导性范例的发展铺平道路。在这种情况下，高分辨率电子显微镜方法，由于它们能够以纳米级分辨率辨别神经形态发生的细节，可能在解开与仿生结构底物接口的神经元的精细超微结构中起关键作用。
