PDF(Pubmed)
Abstract：
Advanced glycation end-products (AGEs) formation increases with metabolic disorders, leading to higher serum AGE levels in patients with progressive vascular complications. Measuring AGE levels in biological samples requires multiple pre-analytical processing steps, rendering analysis of multiple samples challenging. This study evaluated the progression of diabetic complications by analyzing AGE levels using a pre-analytical processing strategy based on a fully automated solid phase-extraction system. Serum samples from patients with diabetes, with or without macrovascular complications (Mac or non-Mac) or microvascular complications (Mic or non-Mic), were processed with the established methods. Free and total AGE levels in sera were measured using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). In patients with diabetes, both free and total AGE levels were elevated in those with complications compared to those without complications. In Mac and Mic groups, free and total AGE levels and z-scores (the sum of normalized AGE levels) also increased. AGE z-scores were markedly higher than those of single AGE levels in distinguishing each complication. Our study demonstrated that the free AGE z-score, measured using a new analytical method without hydrolysis, correlated with the presence of vascular complications and may serve as a marker of disease complications.
摘要：
晚期糖基化终产物（AGEs）的形成随着代谢紊乱而增加，导致进行性血管并发症患者血清AGE水平升高。测量生物样品中的AGE水平需要多个分析前处理步骤，多个样本的渲染分析具有挑战性。这项研究通过基于全自动固相萃取系统的预分析处理策略分析AGE水平来评估糖尿病并发症的进展。糖尿病患者的血清样本,有或没有大血管并发症（Mac或非Mac）或微血管并发症（Mic或非Mic），用既定的方法进行了处理。使用液相色谱与串联质谱联用(LC-MS/MS)测量血清中的游离和总AGE水平。在糖尿病患者中，与无并发症者相比,有并发症者的游离和总AGE水平均升高.在Mac和Mic组中，免费和总AGE水平以及z评分(标准化AGE水平的总和)也增加.在区分每种并发症方面，AGEz评分明显高于单个AGE水平。我们的研究表明，免费的AGEz分数，使用一种没有水解的新分析方法进行测量，与血管并发症的存在相关，可能是疾病并发症的标志。
