Abstract：
Authentication of true (genuine) cow leathers is in high demand to promote merchandise and economic growth. The present study employs RT-PCR-based TaqMan assay to facilitate the identification. Species-specific primers and probes were designed utilizing the existing NCBI data on mitochondrial DNA (mtDNA) genes, particularly the cytochrome b region (Cyt b). Mitochondrial DNA extracted from leather samples of both Bos taurus and Bos indicus and analyzed following the appropriate procedures. The RT-PCR results showed the designed primers and probes are exceptionally precise for cow leather samples. The established detection limit for the assay is estimated as 0.1 ng of DNA. In summary, the amplifiable mtDNA extracted from finished leather enables the identification of authentic cow leathers using the RT-PCR TaqMan assay, representing a pioneering report in this field.
摘要：
对真正的（真正的）牛皮的认证有很高的需求，以促进商品和经济增长。本研究采用基于RT-PCR的TaqMan测定来促进鉴定。利用现有的线粒体DNA（mtDNA）基因的NCBI数据设计物种特异性引物和探针，特别是细胞色素b区(Cytb)。从Bostaurus和Bosindicus的皮革样品中提取的线粒体DNA，并按照适当的程序进行分析。RT-PCR结果表明，设计的引物和探针对牛皮革样品非常精确。确定的测定检测极限估计为0.1ng的DNA。总之,从成品皮革中提取的可扩增的mtDNA可以使用RT-PCRTaqMan测定法鉴定真正的牛皮，代表了这一领域的开创性报告。
