PDF(Pubmed)
Abstract：
Anterior talofibular ligament injuries and osteochondral lesions of the talus present unique challenges to orthopedic surgeons. This study aimed to investigate the relevant relationship between them by analyzing the Magnetic resonance imaging (MRI) results of clinical patients and single-cell RNA sequence (scRNA seq) results of healthy talus cartilage to discuss the risk factors. Data from 164 patients from 2018 to 2023 was retrospectively analyzed. The correlation analysis between ATFL injury grade and the Hepple stage of OLT determined by MRI was performed. Publicly available single-cell RNA datasets were collected. Single-cell RNA datasets from five volunteers of healthy talus cartilage were analyzed. ATFL injury grade was relevant with the Hepple stage of OLT (P < 0.05). The results of multivariate logistic regression analysis showed that injured area was the independent influencing factor of the incidence rate and the severity of OLT (P < 0.05). The Hepple stage of OLT was relevant with AOFAS and VAS (P < 0.05). Single-cell RNA sequence results showed that among the 9 subtypes of chondrocytes, the interaction strength between HTC-A and HTC-B is the highest. Their physical interactions are mainly achieved through the CD99 signaling pathway, and factor interactions are mainly achieved through the ANGPTL signaling pathway. Anterior talofibular ligament injury may lead to osteochondral lesions of the talus. Early medical intervention should be carried out for ligament injuries to restore joint stability and avoid cartilage damage.
摘要：
距腓前韧带损伤和距骨软骨损伤对骨科医生提出了独特的挑战。本研究旨在通过分析临床患者的磁共振成像(MRI)结果和健康距骨软骨的单细胞RNA序列(scRNAseq)结果,探讨两者的相关关系,探讨其危险因素。回顾性分析了2018年至2023年164例患者的数据。对MRI确定的ATFL损伤分级与OLT的Hepple分期进行相关性分析。收集公开可用的单细胞RNA数据集。分析了来自健康距骨软骨的五名志愿者的单细胞RNA数据集。ATFL损伤分级与OLT的Hepple分期有关(P<0.05)。多因素logistic回归分析结果显示,损伤面积是OLT发生率及严重程度的独立影响因素(P<0.05)。OLT的Hepple分期与AOFAS和VAS有关（P<0.05）。单细胞RNA序列结果显示,软骨细胞的9种亚型中,HTC-A和HTC-B之间的相互作用强度最高。它们的物理相互作用主要通过CD99信号通路实现,和因子相互作用主要通过ANGPTL信号通路实现。距腓前韧带损伤可导致距骨软骨损伤。韧带损伤应进行早期医学干预，以恢复关节稳定性，避免软骨损伤。
